6b21

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of AmtB from E. coli bound to TopFluor cardiolipin

Structural highlights

6b21 is a 1 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.45Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

AMTB_ECOLI Involved in the uptake of ammonia.

Publication Abstract from PubMed

Membrane proteins interact with a myriad of lipid species in the biological membrane, leading to a bewildering number of possible protein-lipid assemblies. Despite this inherent complexity, the identification of specific protein-lipid interactions and the crucial role of lipids in the folding, structure, and function of membrane proteins is emerging from an increasing number of reports. Fundamental questions remain, however, regarding the ability of specific lipid binding events to membrane proteins to alter remote binding sites for lipids of a different type, a property referred to as allostery [Monod J, Wyman J, Changeux JP (1965) J Mol Biol 12:88-118]. Here, we use native mass spectrometry to determine the allosteric nature of heterogeneous lipid binding events to membrane proteins. We monitored individual lipid binding events to the ammonia channel (AmtB) from Escherichia coli, enabling determination of their equilibrium binding constants. We found that different lipid pairs display a range of allosteric modulation. In particular, the binding of phosphatidylethanolamine and cardiolipin-like molecules to AmtB exhibited the largest degree of allosteric modulation, inspiring us to determine the cocrystal structure of AmtB in this lipid environment. The 2.45-A resolution structure reveals a cardiolipin-like molecule bound to each subunit of the trimeric complex. Mutation of a single residue in AmtB abolishes the positive allosteric modulation observed for binding phosphatidylethanolamine and cardiolipin-like molecules. Our results demonstrate that specific lipid-protein interactions can act as allosteric modulators for the binding of different lipid types to integral membrane proteins.

Allostery revealed within lipid binding events to membrane proteins.,Patrick JW, Boone CD, Liu W, Conover GM, Liu Y, Cong X, Laganowsky A Proc Natl Acad Sci U S A. 2018 Mar 5. pii: 1719813115. doi:, 10.1073/pnas.1719813115. PMID:29507234^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Patrick JW, Boone CD, Liu W, Conover GM, Liu Y, Cong X, Laganowsky A. Allostery revealed within lipid binding events to membrane proteins. Proc Natl Acad Sci U S A. 2018 Mar 5. pii: 1719813115. doi:, 10.1073/pnas.1719813115. PMID:29507234 doi:http://dx.doi.org/10.1073/pnas.1719813115

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6b21"

Categories: Escherichia coli K-12 | Large Structures | Boone CD | Laganowsky A

@@ Line 1: / Line 1: @@
 ==Crystal structure of AmtB from E. coli bound to TopFluor cardiolipin==
-<StructureSection load='6b21' size='340' side='right' caption='[[6b21]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
+<StructureSection load='6b21' size='340' side='right'caption='[[6b21]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6b21]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B21 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B21 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6b21]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B21 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6B21 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C9V:[(2R,5R,11R,14S,24E)-14-[(acetyloxy)methyl]-8-{[5-(3,5-dimethyl-1H-pyrrol-2-yl-kappaN)-5-(3,5-dimethyl-2H-pyrrol-2-ylidene-kappaN)pentanoyl]oxy}-5,11-dihydroxy-2-{[(9E)-octadec-9-enoyl]oxy}-5,11,16-trioxo-4,6,10,12,15-pentaoxa-5lambda~5~,11lambda~5~-diphosphatritriacont-24-en-1-yl+(9E)-octadec-9-enoatato](difluoro)boron'>C9V</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">amtB, ybaG, b0451, JW0441 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C9V:[(2R,5R,11R,14S,24E)-14-[(acetyloxy)methyl]-8-{[5-(3,5-dimethyl-1H-pyrrol-2-yl-kappaN)-5-(3,5-dimethyl-2H-pyrrol-2-ylidene-kappaN)pentanoyl]oxy}-5,11-dihydroxy-2-{[(9E)-octadec-9-enoyl]oxy}-5,11,16-trioxo-4,6,10,12,15-pentaoxa-5lambda~5~,11lambda~5~-diphosphatritriacont-24-en-1-yl+(9E)-octadec-9-enoatato](difluoro)boron'>C9V</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b21 OCA], [http://pdbe.org/6b21 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b21 RCSB], [http://www.ebi.ac.uk/pdbsum/6b21 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b21 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6b21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b21 OCA], [https://pdbe.org/6b21 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6b21 RCSB], [https://www.ebi.ac.uk/pdbsum/6b21 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6b21 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/AMTB_ECOLI AMTB_ECOLI]] Involved in the uptake of ammonia.
+[https://www.uniprot.org/uniprot/AMTB_ECOLI AMTB_ECOLI] Involved in the uptake of ammonia.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Ecoli]]
+[[Category: Escherichia coli K-12]]
-[[Category: Boone, C D]]
+[[Category: Large Structures]]
-[[Category: Laganowsky, A]]
+[[Category: Boone CD]]
-[[Category: Ammonia channel]]
+[[Category: Laganowsky A]]
-[[Category: Amtb]]
-[[Category: Cardiolipin]]
-[[Category: Membrane protein]]
-[[Category: Transporter]]

6b21

From Proteopedia

Current revision

Crystal structure of AmtB from E. coli bound to TopFluor cardiolipin

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox