6esx

Publication Abstract from PubMed

Thioredoxins (Trxs) are antioxidant proteins that are conserved among all species. These proteins have been extensively studied and perform reducing reactions on a broad range of substrates. Here, we identified Caulobacter crescentus Trx1 (CCNA_03653; CcTrx1) as an oxidoreductase that is involved in the cell cycle progression of this model bacterium and is required to sustain life. Intriguingly, the abundance of CcTrx1 varies throughout the C. crescentus cell cycle: while the expression of CcTrx1 is induced in stalked cells, right before DNA replication initiation, CcTrx1 is actively degraded by the ClpXP protease in predivisional cells. Importantly, we demonstrated that regulation of the abundance of CcTrx1 is crucial for cell growth and survival as modulating CcTrx1 levels leads to cell death. Finally, we also report a comprehensive biochemical and structural characterization of this unique and essential Trx. The requirement to precisely control the abundance of CcTrx1 for cell survival underlines the importance of redox control for optimal cell cycle progression in C. crescentus.

An essential thioredoxin is involved in the control of the cell cycle in the bacterium Caulobacter crescentus.,Goemans CV, Beaufay F, Wahni K, Van Molle I, Messens J, Collet JF J Biol Chem. 2018 Jan 24. pii: RA117.001042. doi: 10.1074/jbc.RA117.001042. PMID:29367337^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.