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| | ==Crystal structure of 3,4-Dihydroxy-2-butanone 4-phosphate Synthase of Candida albicans== | | ==Crystal structure of 3,4-Dihydroxy-2-butanone 4-phosphate Synthase of Candida albicans== |
| - | <StructureSection load='1tks' size='340' side='right' caption='[[1tks]], [[Resolution|resolution]] 1.60Å' scene=''> | + | <StructureSection load='1tks' size='340' side='right'caption='[[1tks]], [[Resolution|resolution]] 1.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1tks]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Canal Canal]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TKS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TKS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1tks]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans_SC5314 Candida albicans SC5314]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TKS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TKS FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1tku|1tku]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CARib3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=237561 CANAL])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tks OCA], [https://pdbe.org/1tks PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tks RCSB], [https://www.ebi.ac.uk/pdbsum/1tks PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tks ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tks OCA], [http://pdbe.org/1tks PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tks RCSB], [http://www.ebi.ac.uk/pdbsum/1tks PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1tks ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/RIB3_CANAL RIB3_CANAL] Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate (By similarity).[HAMAP-Rule:MF_00180] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Canal]] | + | [[Category: Candida albicans SC5314]] |
| - | [[Category: Bacher, A]] | + | [[Category: Large Structures]] |
| - | [[Category: Bauer, S]] | + | [[Category: Bacher A]] |
| - | [[Category: Echt, S]] | + | [[Category: Bauer S]] |
| - | [[Category: Fischer, M]] | + | [[Category: Echt S]] |
| - | [[Category: Huber, R]] | + | [[Category: Fischer M]] |
| - | [[Category: Steinbacher, S]] | + | [[Category: Huber R]] |
| - | [[Category: 4-dihydroxy-2-butanone 4-phosphate synthase]]
| + | [[Category: Steinbacher S]] |
| - | [[Category: Candida albican]]
| + | |
| - | [[Category: Crystal structure synthetic gene]]
| + | |
| - | [[Category: Isomerase]]
| + | |
| - | [[Category: Riboflavin biosynthesis]]
| + | |
| Structural highlights
Function
RIB3_CANAL Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate (By similarity).[HAMAP-Rule:MF_00180]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
A synthetic gene specifying a putative 3,4-dihydroxy-2-butanone 4-phosphate synthase of Candida albicans directed the synthesis of a 22.5 kDa peptide in a recombinant Escherichia coli strain. The recombinant protein was purified to apparent homogeneity by two chromatographic steps and was shown to catalyze the formation of L-3,4-dihydroxy-2-butanone 4-phosphate from ribulose 5-phosphate at a rate of 332 nmol mg(-1) min(-1). Hydrodynamic studies indicated a native molecular mass of 41 kDa in line with a homodimer structure. The protein was crystallized in its apoform. Soaking yielded crystals in complex with the substrate ribulose 5-phosphate. The structures were solved at resolutions of 1.6 and 1.7 angstroms, respectively, using 3,4-dihydroxy-2-butanone 4-phosphate synthase of E. coli for molecular replacement. Structural comparison with the orthologs of Magnaporthe grisea and Methanococcus jannaschii revealed a hitherto unknown conformation of the essential acidic active-site loop.
Potential anti-infective targets in pathogenic yeasts: structure and properties of 3,4-dihydroxy-2-butanone 4-phosphate synthase of Candida albicans.,Echt S, Bauer S, Steinbacher S, Huber R, Bacher A, Fischer M J Mol Biol. 2004 Aug 20;341(4):1085-96. PMID:15328619[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Echt S, Bauer S, Steinbacher S, Huber R, Bacher A, Fischer M. Potential anti-infective targets in pathogenic yeasts: structure and properties of 3,4-dihydroxy-2-butanone 4-phosphate synthase of Candida albicans. J Mol Biol. 2004 Aug 20;341(4):1085-96. PMID:15328619 doi:10.1016/j.jmb.2004.06.053
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