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| | ==Crystal Structure of the Alginate Lyase from Corynebacterium sp.== | | ==Crystal Structure of the Alginate Lyase from Corynebacterium sp.== |
| - | <StructureSection load='1uai' size='340' side='right' caption='[[1uai]], [[Resolution|resolution]] 1.20Å' scene=''> | + | <StructureSection load='1uai' size='340' side='right'caption='[[1uai]], [[Resolution|resolution]] 1.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1uai]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Corynebacterium_sp. Corynebacterium sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UAI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UAI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1uai]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_sp. Corynebacterium sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UAI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UAI FirstGlance]. <br> |
| - | </td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Poly(alpha-L-guluronate)_lyase Poly(alpha-L-guluronate) lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.11 4.2.2.11] </span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uai FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uai OCA], [http://pdbe.org/1uai PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1uai RCSB], [http://www.ebi.ac.uk/pdbsum/1uai PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1uai ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uai FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uai OCA], [https://pdbe.org/1uai PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uai RCSB], [https://www.ebi.ac.uk/pdbsum/1uai PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uai ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9RB42_9CORY Q9RB42_9CORY] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Corynebacterium sp]] | | [[Category: Corynebacterium sp]] |
| - | [[Category: Kakuta, Y]] | + | [[Category: Large Structures]] |
| - | [[Category: Jellyroll beta-sandwich]] | + | [[Category: Kakuta Y]] |
| - | [[Category: Lyase]]
| + | |
| Structural highlights
Function
Q9RB42_9CORY
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The crystal structure of alginate (poly alpha-l-guluronate) lyase from Corynebacterium sp. (ALY-1) was determined at 1.2A resolution using the MAD method and bromide ions. The structure of ALY-1 is abundant in beta-strands and has a deep cleft, similar to the jellyroll beta-sandwich found in 1,3-1,4-beta-glucanase. The structure suggests that alginate molecules may penetrate into the cleft to interact with the catalytic site of ALY-1. The reported crystal structure of another type of alginate lyase, A1-III, differs from that of ALY-1 in that it consists almost entirely of alpha-helical structure. Nevertheless, the putative catalytic residues in both enzymes are positioned in space in nearly identical arrangements. This finding suggests that both alginate lyases may have evolved through convergent evolution.
Crystal structure of the alginate (poly alpha-l-guluronate) lyase from Corynebacterium sp. at 1.2 A resolution.,Osawa T, Matsubara Y, Muramatsu T, Kimura M, Kakuta Y J Mol Biol. 2005 Feb 4;345(5):1111-8. Epub 2004 Dec 13. PMID:15644208[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Osawa T, Matsubara Y, Muramatsu T, Kimura M, Kakuta Y. Crystal structure of the alginate (poly alpha-l-guluronate) lyase from Corynebacterium sp. at 1.2 A resolution. J Mol Biol. 2005 Feb 4;345(5):1111-8. Epub 2004 Dec 13. PMID:15644208 doi:http://dx.doi.org/10.1016/j.jmb.2004.10.081
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