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| - | [[Image:2g23.gif|left|200px]] | + | #REDIRECT [[3gyr]] This PDB entry is obsolete and replaced by 3gyr |
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| - | {{Structure
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| - | |PDB= 2g23 |SIZE=350|CAPTION= <scene name='initialview01'>2g23</scene>, resolution 2.30Å
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| - | |SITE=
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| - | |LIGAND= <scene name='pdbligand=C2O:CU-O-CU+LINKAGE'>C2O</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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| - | |ACTIVITY=
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| - | |GENE= phsA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1890 Streptomyces antibioticus])
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| - | |DOMAIN=
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| - | |RELATEDENTRY=
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| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g23 OCA], [http://www.ebi.ac.uk/pdbsum/2g23 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2g23 RCSB]</span>
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| - | }}
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| - | '''The crystal structure of hexameric phenoxazinone synthase'''
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| - | ==Overview==
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| - | The multicopper oxidase phenoxazinone synthase (PHS) catalyzes the penultimate step in the biosynthesis of the antibiotic actinomycin D by Streptomyces antibioticus. PHS exists in two oligomeric forms: a dimeric form and a hexameric form, with older actinomycin-producing cultures containing predominately the hexameric form. The structure of hexameric PHS has been determined using X-ray diffraction to a resolution limit of 2.30 A and is found to contain several unexpected and distinctive features. The structure forms a hexameric ring that is centered on a pseudo 6-fold axis and has an outer diameter of 185 A with a large central cavity that has a diameter of 50 A. This hexameric structure is stabilized by a long loop connecting two domains; bound to this long loop is a fifth copper atom that is present as a type 2 copper. This copper atom is not present in any other multicopper oxidase, and its presence appears to stabilize the hexameric structure.
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| - | ==About this Structure==
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| - | 2G23 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_antibioticus Streptomyces antibioticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G23 OCA].
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| - | ==Reference==
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| - | Structure of phenoxazinone synthase from Streptomyces antibioticus reveals a new type 2 copper center., Smith AW, Camara-Artigas A, Wang M, Allen JP, Francisco WA, Biochemistry. 2006 Apr 11;45(14):4378-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16584173 16584173]
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| - | [[Category: Single protein]]
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| - | [[Category: Streptomyces antibioticus]]
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| - | [[Category: Allen, J P.]]
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| - | [[Category: Camara-Artigas, A.]]
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| - | [[Category: Francisco, W A.]]
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| - | [[Category: Smith, A W.]]
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| - | [[Category: Wang, M.]]
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| - | [[Category: copper]]
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| - | [[Category: hexamer]]
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| - | [[Category: laccase]]
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| - | [[Category: metalloprotein]]
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| - | [[Category: multicopper oxidase]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:10:47 2008''
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