5zdl
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure Analysis of TtQRS in co-crystallised with ATP== | |
| + | <StructureSection load='5zdl' size='340' side='right'caption='[[5zdl]], [[Resolution|resolution]] 2.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5zdl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZDL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZDL FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zdl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zdl OCA], [https://pdbe.org/5zdl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zdl RCSB], [https://www.ebi.ac.uk/pdbsum/5zdl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zdl ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q5SKU4_THET8 Q5SKU4_THET8] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Aminoacyl-tRNA synthetases (AaRSs) are vital enzymes for translation of proteins in cells. AaRSs catalyse the esterification of a specific amino acid to corresponding tRNAs to form an aminoacyl-tRNA that is used in ribosome-based protein synthesis. We focused on Glutaminyl tRNA synthetase (GlnRS) enzyme from the extreme thermophile Thermus thermophilus for structural studies. Our thermal shift assays show binding of enzyme substrates L-Gln and ATP as well as of various metals including cesium. We resolved crystal structures of apo-GlnRS as well as those in complex with AMP and ATP at 2.8A, 2.4A and 2.6A respectively. The bound cesium was found at the site of magnesium that typically binds to GlnRS. High structural conservation was evident in the Thermus thermophilus GlnRS when compared to those from Escherichia coli GlnRS. | ||
| - | + | Structural and functional analysis of Glutaminyl-tRNA synthetase (TtGlnRS) from Thermus thermophilus HB8 and its complexes.,Nachiappan M, Jain V, Sharma A, Yogavel M, Jeyakanthan J Int J Biol Macromol. 2018 Dec;120(Pt B):1379-1386. doi:, 10.1016/j.ijbiomac.2018.09.115. Epub 2018 Sep 21. PMID:30248426<ref>PMID:30248426</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5zdl" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: Jeyaraman | + | ==See Also== |
| - | [[Category: Mutharasappan | + | *[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]] |
| - | [[Category: Sharma | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Thermus thermophilus HB8]] | ||
| + | [[Category: Jain V]] | ||
| + | [[Category: Jeyaraman J]] | ||
| + | [[Category: Manickam Y]] | ||
| + | [[Category: Mutharasappan N]] | ||
| + | [[Category: Sharma A]] | ||
Current revision
Crystal Structure Analysis of TtQRS in co-crystallised with ATP
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