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2g4i

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[[Image:2g4i.gif|left|200px]]
 
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{{Structure
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==Anomalous substructure of Concanavalin A==
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|PDB= 2g4i |SIZE=350|CAPTION= <scene name='initialview01'>2g4i</scene>, resolution 2.40&Aring;
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<StructureSection load='2g4i' size='340' side='right'caption='[[2g4i]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
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<table><tr><td colspan='2'>[[2g4i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Canavalia_cathartica Canavalia cathartica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G4I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G4I FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g4i OCA], [https://pdbe.org/2g4i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g4i RCSB], [https://www.ebi.ac.uk/pdbsum/2g4i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g4i ProSAT]</span></td></tr>
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|RELATEDENTRY=[[2g4h|2G4H]], [[2g4j|2G4J]], [[2g4k|2G4K]], [[2g4l|2G4L]], [[2g4m|2G4M]], [[2g4n|2G4N]], [[2g4o|2G4O]], [[2g4p|2G4P]], [[2g4q|2G4Q]], [[2g4r|2G4R]], [[2g4s|2G4S]], [[2g4t|2G4T]], [[2g4u|2G4U]], [[2g4v|2G4V]], [[2g4w|2G4W]], [[2g4x|2G4X]], [[2g4y|2G4Y]], [[2g4z|2G4Z]], [[2g51|2G51]], [[2g52|2G52]], [[2g55|2G55]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g4i OCA], [http://www.ebi.ac.uk/pdbsum/2g4i PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2g4i RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/CONA_CANCT CONA_CANCT] Glucose/D-mannose specific lectin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g4/2g4i_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2g4i ConSurf].
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<div style="clear:both"></div>
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'''Anomalous substructure of Concanavalin A'''
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==See Also==
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*[[Concanavalin 3D structures|Concanavalin 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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23 different crystal forms of 19 different biological macromolecules were examined with respect to their anomalously scattering substructures using diffraction data collected at a wavelength of 2.0 A (6.2 keV). In more than 90% of the cases the substructure was found to contain more than just the protein S atoms. The data presented suggest that chloride, sulfate, phosphate or metal ions from the buffer or even from the purification protocol are frequently bound to the protein molecule and that these ions are often overlooked, especially if they are not bound at full occupancy. Thus, in order to fully describe the macromolecule under study, it seems desirable that any structure determination be complemented with a long-wavelength data set.
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[[Category: Canavalia cathartica]]
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[[Category: Large Structures]]
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==About this Structure==
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[[Category: Mueller-Dieckmann C]]
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2G4I is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Canavalia_virosa Canavalia virosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G4I OCA].
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[[Category: Weiss MS]]
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==Reference==
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On the routine use of soft X-rays in macromolecular crystallography. Part IV. Efficient determination of anomalous substructures in biomacromolecules using longer X-ray wavelengths., Mueller-Dieckmann C, Panjikar S, Schmidt A, Mueller S, Kuper J, Geerlof A, Wilmanns M, Singh RK, Tucker PA, Weiss MS, Acta Crystallogr D Biol Crystallogr. 2007 Mar;63(Pt 3):366-80. Epub 2007, Feb 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17327674 17327674]
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[[Category: Canavalia virosa]]
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[[Category: Single protein]]
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[[Category: Mueller-Dieckmann, C.]]
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[[Category: Weiss, M S.]]
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[[Category: anomalous substructure of concanavalin some]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:11:40 2008''
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Current revision

Anomalous substructure of Concanavalin A

PDB ID 2g4i

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