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6cr0
From Proteopedia
(Difference between revisions)
(New page: ==1.55 A resolution structure of (S)-6-hydroxynicotine oxidase from Shinella HZN7== <StructureSection load='6cr0' size='340' side='right' caption='6cr0, resolution 1.55...) |
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==1.55 A resolution structure of (S)-6-hydroxynicotine oxidase from Shinella HZN7== | ==1.55 A resolution structure of (S)-6-hydroxynicotine oxidase from Shinella HZN7== | ||
| - | <StructureSection load='6cr0' size='340' side='right' caption='[[6cr0]], [[Resolution|resolution]] 1.55Å' scene=''> | + | <StructureSection load='6cr0' size='340' side='right'caption='[[6cr0]], [[Resolution|resolution]] 1.55Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6cr0]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CR0 OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[6cr0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Shinella_sp._HZN7 Shinella sp. HZN7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CR0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CR0 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.548Å</td></tr> |
| - | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr> | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cr0 OCA], [https://pdbe.org/6cr0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cr0 RCSB], [https://www.ebi.ac.uk/pdbsum/6cr0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cr0 ProSAT]</span></td></tr> |
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/HLNO_SHIS7 HLNO_SHIS7] Involved in the degradation of L-nicotine (PubMed:25002425). Catalyzes the oxidation of (S)-6-hydroxynicotine (6-hydroxy-L-nicotine) to 6-hydroxypseudooxynicotine (PubMed:25002425). Oxidation of the pyrrolidine ring of (S)-6-hydroxynicotine leads to the formation of the optically inactive 6-hydroxy-N-methylmyosmine, which hydrolyzes spontaneously to 6-hydroxypseudooxynicotine (PubMed:25002425). Acts with absolute stereospecificity on the L-form of 6-hydroxynicotine (PubMed:25002425). Also involved in the degradation of nornicotine, and catalyzes the oxidation of 6-hydroxynornicotine to 6-hydroxymyosmine, which hydrolyzes to 6-hydroxypseudooxynornicotine (PubMed:27568381). In vitro, converts (S)-nicotine into N-methylmyosmine, which spontaneously hydrolyzes spontaneously into pseudooxynicotine, but catalytic efficiency is about 1900-fold higher with (S)-6-hydroxynicotine (PubMed:25002425).<ref>PMID:25002425</ref> <ref>PMID:27568381</ref> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Shinella sp. HZN7]] |
| - | [[Category: | + | [[Category: Battaile KP]] |
| - | [[Category: | + | [[Category: Deay III D]] |
| - | [[Category: | + | [[Category: Lovell S]] |
| - | [[Category: | + | [[Category: Petillo P]] |
| - | [[Category: | + | [[Category: Richter M]] |
| - | + | ||
Current revision
1.55 A resolution structure of (S)-6-hydroxynicotine oxidase from Shinella HZN7
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