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| | ==Spliceosome component== | | ==Spliceosome component== |
| - | <StructureSection load='5m88' size='340' side='right' caption='[[5m88]], [[Resolution|resolution]] 2.80Å' scene=''> | + | <StructureSection load='5m88' size='340' side='right'caption='[[5m88]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5m88]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chatd Chatd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M88 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5M88 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5m88]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M88 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5M88 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CTHT_0072540 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5m88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m88 OCA], [http://pdbe.org/5m88 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m88 RCSB], [http://www.ebi.ac.uk/pdbsum/5m88 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m88 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5m88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m88 OCA], [https://pdbe.org/5m88 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5m88 RCSB], [https://www.ebi.ac.uk/pdbsum/5m88 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5m88 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/G0SFY0_CHATD G0SFY0_CHATD] |
| - | Human nineteen complex (NTC) acts as a multimeric E3 ubiquitin ligase in DNA repair and splicing. The transfer of ubiquitin is mediated by Prp19-a homotetrameric component of NTC whose elongated coiled coils serve as an assembly axis for two other proteins called SPF27 and CDC5L. We find that Prp19 is inactive on its own and have elucidated the structural basis of its autoinhibition by crystallography and mutational analysis. Formation of the NTC core by stepwise assembly of SPF27, CDC5L, and PLRG1 onto the Prp19 tetramer enables ubiquitin ligation. Protein-protein crosslinking of NTC, functional assays in vitro, and assessment of its role in DNA damage response provide mechanistic insight into the organization of the NTC core and the communication between PLRG1 and Prp19 that enables E3 activity. This reveals a unique mode of regulation for a complex E3 ligase and advances understanding of its dynamics in various cellular pathways.
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| - | Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors.,de Moura TR, Mozaffari-Jovin S, Szabo CZK, Schmitzova J, Dybkov O, Cretu C, Kachala M, Svergun D, Urlaub H, Luhrmann R, Pena V Mol Cell. 2018 Mar 15;69(6):979-992.e6. doi: 10.1016/j.molcel.2018.02.022. PMID:29547724<ref>PMID:29547724</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 5m88" style="background-color:#fffaf0;"></div>
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| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Chatd]] | + | [[Category: Chaetomium thermophilum var. thermophilum DSM 1495]] |
| - | [[Category: Moura, T R]] | + | [[Category: Large Structures]] |
| - | [[Category: Pena, V]] | + | [[Category: Moura TR]] |
| - | [[Category: Spliceosome]] | + | [[Category: Pena V]] |
| - | [[Category: Splicing]]
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