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| | ==Structure of the SNX5 PX domain in complex with chlamydial protein IncE in space group P32== | | ==Structure of the SNX5 PX domain in complex with chlamydial protein IncE in space group P32== |
| - | <StructureSection load='5tgh' size='340' side='right' caption='[[5tgh]], [[Resolution|resolution]] 2.80Å' scene=''> | + | <StructureSection load='5tgh' size='340' side='right'caption='[[5tgh]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5tgh]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"chlamydozoon_trachomatis"_(busacca_1935)_moshkovski_1945 "chlamydozoon trachomatis" (busacca 1935) moshkovski 1945] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TGH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TGH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5tgh]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydia_trachomatis Chlamydia trachomatis] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TGH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TGH FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5tgj|5tgj]], [[5tgi|5tgi]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SNX5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), incE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=813 "Chlamydozoon trachomatis" (Busacca 1935) Moshkovski 1945])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tgh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tgh OCA], [https://pdbe.org/5tgh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tgh RCSB], [https://www.ebi.ac.uk/pdbsum/5tgh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tgh ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5tgh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tgh OCA], [http://pdbe.org/5tgh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tgh RCSB], [http://www.ebi.ac.uk/pdbsum/5tgh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tgh ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SNX5_HUMAN SNX5_HUMAN]] May be involved in several stages of intracellular trafficking. Plays a role in macropinocytosis. Plays a role in the internalization of EGFR after EGF stimulation.<ref>PMID:18854019</ref> <ref>PMID:21048941</ref> | + | [https://www.uniprot.org/uniprot/SNX5_HUMAN SNX5_HUMAN] May be involved in several stages of intracellular trafficking. Plays a role in macropinocytosis. Plays a role in the internalization of EGFR after EGF stimulation.<ref>PMID:18854019</ref> <ref>PMID:21048941</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Sorting nexin|Sorting nexin]] | + | *[[Sorting nexin 3D structures|Sorting nexin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Chlamydia trachomatis]] |
| - | [[Category: Collins, B]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Paul, B]] | + | [[Category: Large Structures]] |
| - | [[Category: Host-pathogen endosome]] | + | [[Category: Collins B]] |
| - | [[Category: Protein transport]] | + | [[Category: Paul B]] |
| Structural highlights
Function
SNX5_HUMAN May be involved in several stages of intracellular trafficking. Plays a role in macropinocytosis. Plays a role in the internalization of EGFR after EGF stimulation.[1] [2]
Publication Abstract from PubMed
During infection chlamydial pathogens form an intracellular membrane-bound replicative niche termed the inclusion, which is enriched with bacterial transmembrane proteins called Incs. Incs bind and manipulate host cell proteins to promote inclusion expansion and provide camouflage against innate immune responses. Sorting nexin (SNX) proteins that normally function in endosomal membrane trafficking are a major class of inclusion-associated host proteins, and are recruited by IncE/CT116. Crystal structures of the SNX5 phox-homology (PX) domain in complex with IncE define the precise molecular basis for these interactions. The binding site is unique to SNX5 and related family members SNX6 and SNX32. Intriguingly the site is also conserved in SNX5 homologues throughout evolution, suggesting that IncE captures SNX5-related proteins by mimicking a native host protein interaction. These findings thus provide the first mechanistic insights both into how chlamydial Incs hijack host proteins, and how SNX5-related PX domains function as scaffolds in protein complex assembly.
Structural basis for the hijacking of endosomal sorting nexin proteins by Chlamydia trachomatis.,Paul B, Kim HS, Kerr MC, Huston WM, Teasdale RD, Collins BM Elife. 2017 Feb 22;6. pii: e22311. doi: 10.7554/eLife.22311. PMID:28226239[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Lim JP, Wang JT, Kerr MC, Teasdale RD, Gleeson PA. A role for SNX5 in the regulation of macropinocytosis. BMC Cell Biol. 2008 Oct 14;9:58. doi: 10.1186/1471-2121-9-58. PMID:18854019 doi:10.1186/1471-2121-9-58
- ↑ Wang JT, Kerr MC, Karunaratne S, Jeanes A, Yap AS, Teasdale RD. The SNX-PX-BAR family in macropinocytosis: the regulation of macropinosome formation by SNX-PX-BAR proteins. PLoS One. 2010 Oct 29;5(10):e13763. doi: 10.1371/journal.pone.0013763. PMID:21048941 doi:10.1371/journal.pone.0013763
- ↑ Paul B, Kim HS, Kerr MC, Huston WM, Teasdale RD, Collins BM. Structural basis for the hijacking of endosomal sorting nexin proteins by Chlamydia trachomatis. Elife. 2017 Feb 22;6. pii: e22311. doi: 10.7554/eLife.22311. PMID:28226239 doi:http://dx.doi.org/10.7554/eLife.22311
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