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2g9w
From Proteopedia
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| - | [[Image:2g9w.jpg|left|200px]] | ||
| - | + | ==Crystal Structure of Rv1846c, a Putative Transcriptional Regulatory Protein of Mycobacterium Tuberculosis== | |
| - | + | <StructureSection load='2g9w' size='340' side='right'caption='[[2g9w]], [[Resolution|resolution]] 1.80Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | | | + | <table><tr><td colspan='2'>[[2g9w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G9W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G9W FirstGlance]. <br> |
| - | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | |
| - | | | + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Rv1846c ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr> | |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g9w OCA], [https://pdbe.org/2g9w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g9w RCSB], [https://www.ebi.ac.uk/pdbsum/2g9w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g9w ProSAT]</span></td></tr> | |
| - | + | </table> | |
| - | + | == Function == | |
| + | [[https://www.uniprot.org/uniprot/BLAI_MYCTO BLAI_MYCTO]] Transcription regulator that regulates genes involved in antibiotic transport, detoxification and cell wall function. Also regulates its own transcription. Binds DNA as a dimer (By similarity). | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g9/2g9w_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2g9w ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Comparative genomics with Staphylococcus aureus suggested the existence of a regulatory system governing beta-lactamase (BlaC) production in Mycobacterium tuberculosis. The crystal structure of Rv1846c, a winged helix regulator of previously unknown function, was solved thus revealing strong similarity to the BlaI and MecI repressors of S. aureus, which both respond to beta-lactam treatment. Using chromatin immunoprecipitation and hybridization to microarrays (ChIP-on-chip), the Rv1846c regulon was shown to comprise five separate genomic loci. Two of these mediate responses and resistance to beta-lactam antibiotics (rv1845c, rv1846c-rv1847; blaC-sigC); two encode membrane proteins of unknown function (rv1456c, rv3921c) while the last codes for ATP synthase (rv1303-atpBEFHAGDC-rv1312). The ChIP-on-chip findings were confirmed independently using electrophoretic mobility shift assays, DNAse footprinting and transcript analysis leading to Rv1846c being renamed BlaI. When cells were treated with beta-lactams, BlaI was released from its operator sites causing derepression of the regulon and upregulation of ATP synthase transcription. The existence of a potential regulatory loop between cell wall integrity and ATP production was previously unknown. | ||
| - | + | Genome-wide regulon and crystal structure of BlaI (Rv1846c) from Mycobacterium tuberculosis.,Sala C, Haouz A, Saul FA, Miras I, Rosenkrands I, Alzari PM, Cole ST Mol Microbiol. 2009 Mar;71(5):1102-16. Epub 2009 Jan 16. PMID:19154333<ref>PMID:19154333</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 2g9w" style="background-color:#fffaf0;"></div> | |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: Alzari, P M | + | __TOC__ |
| - | [[Category: Fiez-Vandal, C | + | </StructureSection> |
| - | [[Category: Haouz, A | + | [[Category: Large Structures]] |
| - | [[Category: Saul, F A | + | [[Category: Myctu]] |
| - | [[Category: Shepard, W | + | [[Category: Alzari, P M]] |
| - | [[Category: | + | [[Category: Fiez-Vandal, C]] |
| - | [[Category: | + | [[Category: Haouz, A]] |
| - | + | [[Category: Saul, F A]] | |
| - | + | [[Category: Shepard, W]] | |
| + | [[Category: Bacterial transcription repressor]] | ||
| + | [[Category: Dna binding protein]] | ||
| + | [[Category: Dna-binding domain]] | ||
Current revision
Crystal Structure of Rv1846c, a Putative Transcriptional Regulatory Protein of Mycobacterium Tuberculosis
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