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| - | [[Image:2gcs.gif|left|200px]] | |
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| - | {{Structure
| + | ==Pre-cleavage state of the Thermoanaerobacter tengcongensis glmS ribozyme== |
| - | |PDB= 2gcs |SIZE=350|CAPTION= <scene name='initialview01'>2gcs</scene>, resolution 2.10Å
| + | <StructureSection load='2gcs' size='340' side='right'caption='[[2gcs]], [[Resolution|resolution]] 2.10Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=2AD:2'-AMINO-2'-DEOXYADENOSINE'>2AD</scene>, <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=A23:ADENOSINE-5'-PHOSPHATE-2',3'-CYCLIC+PHOSPHATE'>A23</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene>
| + | <table><tr><td colspan='2'>[[2gcs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus_subsp._tengcongensis Caldanaerobacter subterraneus subsp. tengcongensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GCS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GCS FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2AD:2-AMINO-2-DEOXYADENOSINE'>2AD</scene>, <scene name='pdbligand=A23:ADENOSINE-5-PHOSPHATE-2,3-CYCLIC+PHOSPHATE'>A23</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gcs OCA], [https://pdbe.org/2gcs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gcs RCSB], [https://www.ebi.ac.uk/pdbsum/2gcs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gcs ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=[[2gcv|2GCV]], [[2h0s|2H0S]], [[2h0w|2H0W]], [[2h0x|2H0X]], [[2h0z|2H0Z]]
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gcs OCA], [http://www.ebi.ac.uk/pdbsum/2gcs PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gcs RCSB]</span>
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| - | }}
| + | |
| | | | |
| - | '''Pre-cleavage state of the Thermoanaerobacter tengcongensis glmS ribozyme'''
| + | ==See Also== |
| - | | + | *[[Ribozyme 3D structures|Ribozyme 3D structures]] |
| - | | + | __TOC__ |
| - | ==Overview== | + | </StructureSection> |
| - | The glmS ribozyme is the only natural catalytic RNA known to require a small-molecule activator for catalysis. This catalytic RNA functions as a riboswitch, with activator-dependent RNA cleavage regulating glmS messenger RNA expression. We report crystal structures of the glmS ribozyme in precleavage states that are unliganded or bound to the competitive inhibitor glucose-6-phosphate and in the postcleavage state. All structures superimpose closely, revealing a remarkably rigid RNA that contains a preformed active and coenzyme-binding site. Unlike other riboswitches, the glmS ribozyme binds its activator in an open, solvent-accessible pocket. Our structures suggest that the amine group of the glmS ribozyme-bound coenzyme performs general acid-base and electrostatic catalysis.
| + | [[Category: Caldanaerobacter subterraneus subsp. tengcongensis]] |
| - | | + | [[Category: Large Structures]] |
| - | ==About this Structure==
| + | [[Category: Ferre-D'Amare AR]] |
| - | 2GCS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GCS OCA].
| + | [[Category: Klein DJ]] |
| - | | + | |
| - | ==Reference==
| + | |
| - | Structural basis of glmS ribozyme activation by glucosamine-6-phosphate., Klein DJ, Ferre-D'Amare AR, Science. 2006 Sep 22;313(5794):1752-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16990543 16990543]
| + | |
| - | [[Category: Single protein]] | + | |
| - | [[Category: Amare, A R.Ferre-D.]] | + | |
| - | [[Category: Klein, D J.]] | + | |
| - | [[Category: helix]]
| + | |
| - | [[Category: pseudoknot]]
| + | |
| - | [[Category: ribozyme]]
| + | |
| - | [[Category: rna]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:15:06 2008''
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