2gfi

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[[Image:2gfi.gif|left|200px]]
 
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{{Structure
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==Crystal structure of the phytase from D. castellii at 2.3 A==
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|PDB= 2gfi |SIZE=350|CAPTION= <scene name='initialview01'>2gfi</scene>, resolution 2.290&Aring;
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<StructureSection load='2gfi' size='340' side='right'caption='[[2gfi]], [[Resolution|resolution]] 2.29&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>
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<table><tr><td colspan='2'>[[2gfi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Debaryomyces_castellii Debaryomyces castellii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GFI FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/3-phytase 3-phytase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.8 3.1.3.8] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.29&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gfi OCA], [https://pdbe.org/2gfi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gfi RCSB], [https://www.ebi.ac.uk/pdbsum/2gfi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gfi ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gfi OCA], [http://www.ebi.ac.uk/pdbsum/2gfi PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gfi RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/A2TBB4_9ASCO A2TBB4_9ASCO]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gf/2gfi_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gfi ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Phytate (myo-inositol hexakisphosphate) is the primary storage form of phosphate in seeds and legumes (Reddy et al., 1982). Phytases are phosphatases that hydrolyze phytate to less phosphorylated myo-inositol derivatives and inorganic phosphate. The crystal structure of phytase from Debaryomyces castellii has been determined at 2.3 A resolution. The crystals belonged to space group P6(5)22, with unit-cell parameters a = 121.65, c = 332.24 A. The structure was solved by molecular replacement and refined to a final R factor of 15.7% (R(free) = 20.9%). The final model consists of a dimer (with two monomers of 458 residues), five NAG molecules and 628 water molecules.
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'''Crystal structure of the phytase from D. castellii at 2.3 A'''
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Structure of Debaryomyces castellii CBS 2923 phytase.,Ragon M, Hoh F, Aumelas A, Chiche L, Moulin G, Boze H Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Apr 1;65(Pt, 4):321-6. Epub 2009 Mar 25. PMID:19342770<ref>PMID:19342770</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2gfi" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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2GFI is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Debaryomyces_castellii Debaryomyces castellii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GFI OCA].
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*[[Phytase 3D structures|Phytase 3D structures]]
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[[Category: 3-phytase]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Debaryomyces castellii]]
[[Category: Debaryomyces castellii]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Hoh, F.]]
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[[Category: Hoh F]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:15:58 2008''
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Current revision

Crystal structure of the phytase from D. castellii at 2.3 A

PDB ID 2gfi

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