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| - | [[Image:2gfx.gif|left|200px]] | |
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| - | {{Structure
| + | ==Structure of E. coli FabF(C163Q) in complex with Platensimycin== |
| - | |PDB= 2gfx |SIZE=350|CAPTION= <scene name='initialview01'>2gfx</scene>, resolution 2.59Å
| + | <StructureSection load='2gfx' size='340' side='right'caption='[[2gfx]], [[Resolution|resolution]] 2.59Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=PMN:PLATENSIMYCIN'>PMN</scene>
| + | <table><tr><td colspan='2'>[[2gfx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GFX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GFX FirstGlance]. <br> |
| - | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-ketoacyl-acyl-carrier-protein_synthase_I Beta-ketoacyl-acyl-carrier-protein synthase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41] </span>
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.59Å</td></tr> |
| - | |GENE= fabF, fabJ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PMN:PLATENSIMYCIN'>PMN</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gfx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gfx OCA], [https://pdbe.org/2gfx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gfx RCSB], [https://www.ebi.ac.uk/pdbsum/2gfx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gfx ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=[[2gfv|2GFV]], [[2gfw|2GFW]], [[2gfy|2GFY]]
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gfx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gfx OCA], [http://www.ebi.ac.uk/pdbsum/2gfx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gfx RCSB]</span>
| + | == Function == |
| - | }}
| + | [https://www.uniprot.org/uniprot/FABF_ECOLI FABF_ECOLI] Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Has a preference for short chain acid substrates and may function to supply the octanoic substrates for lipoic acid biosynthesis. |
| | + | == Evolutionary Conservation == |
| | + | [[Image:Consurf_key_small.gif|200px|right]] |
| | + | Check<jmol> |
| | + | <jmolCheckbox> |
| | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gf/2gfx_consurf.spt"</scriptWhenChecked> |
| | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | + | <text>to colour the structure by Evolutionary Conservation</text> |
| | + | </jmolCheckbox> |
| | + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gfx ConSurf]. |
| | + | <div style="clear:both"></div> |
| | | | |
| - | '''Structure of E. coli FabF(C163Q) in complex with Platensimycin'''
| + | ==See Also== |
| - | | + | *[[Acyl carrier protein synthase 3D structures|Acyl carrier protein synthase 3D structures]] |
| - | | + | __TOC__ |
| - | ==Overview== | + | </StructureSection> |
| - | Bacterial infection remains a serious threat to human lives because of emerging resistance to existing antibiotics. Although the scientific community has avidly pursued the discovery of new antibiotics that interact with new targets, these efforts have met with limited success since the early 1960s. Here we report the discovery of platensimycin, a previously unknown class of antibiotics produced by Streptomyces platensis. Platensimycin demonstrates strong, broad-spectrum Gram-positive antibacterial activity by selectively inhibiting cellular lipid biosynthesis. We show that this anti-bacterial effect is exerted through the selective targeting of beta-ketoacyl-(acyl-carrier-protein (ACP)) synthase I/II (FabF/B) in the synthetic pathway of fatty acids. Direct binding assays show that platensimycin interacts specifically with the acyl-enzyme intermediate of the target protein, and X-ray crystallographic studies reveal that a specific conformational change that occurs on acylation must take place before the inhibitor can bind. Treatment with platensimycin eradicates Staphylococcus aureus infection in mice. Because of its unique mode of action, platensimycin shows no cross-resistance to other key antibiotic-resistant strains tested, including methicillin-resistant S. aureus, vancomycin-intermediate S. aureus and vancomycin-resistant enterococci. Platensimycin is the most potent inhibitor reported for the FabF/B condensing enzymes, and is the only inhibitor of these targets that shows broad-spectrum activity, in vivo efficacy and no observed toxicity.
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| - | | + | |
| - | ==About this Structure==
| + | |
| - | 2GFX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GFX OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | Platensimycin is a selective FabF inhibitor with potent antibiotic properties., Wang J, Soisson SM, Young K, Shoop W, Kodali S, Galgoci A, Painter R, Parthasarathy G, Tang YS, Cummings R, Ha S, Dorso K, Motyl M, Jayasuriya H, Ondeyka J, Herath K, Zhang C, Hernandez L, Allocco J, Basilio A, Tormo JR, Genilloud O, Vicente F, Pelaez F, Colwell L, Lee SH, Michael B, Felcetto T, Gill C, Silver LL, Hermes JD, Bartizal K, Barrett J, Schmatz D, Becker JW, Cully D, Singh SB, Nature. 2006 May 18;441(7091):358-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16710421 16710421]
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| - | [[Category: Beta-ketoacyl-acyl-carrier-protein synthase I]]
| + | |
| | [[Category: Escherichia coli]] | | [[Category: Escherichia coli]] |
| - | [[Category: Single protein]] | + | [[Category: Large Structures]] |
| - | [[Category: Parthasarathy, G.]] | + | [[Category: Parthasarathy G]] |
| - | [[Category: Soisson, S M.]] | + | [[Category: Soisson SM]] |
| - | [[Category: fabf]]
| + | |
| - | [[Category: kasii]]
| + | |
| - | [[Category: ketoacyl synthase]]
| + | |
| - | [[Category: platensimycin]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:16:03 2008''
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