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| | ==LIPOXYGENASE-3 (SOYBEAN) COMPLEX WITH 4-HYDROPEROXY-2-METHOXY-PHENOL== | | ==LIPOXYGENASE-3 (SOYBEAN) COMPLEX WITH 4-HYDROPEROXY-2-METHOXY-PHENOL== |
| - | <StructureSection load='1hu9' size='340' side='right' caption='[[1hu9]], [[Resolution|resolution]] 2.20Å' scene=''> | + | <StructureSection load='1hu9' size='340' side='right'caption='[[1hu9]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1hu9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Glycine_max Glycine max]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HU9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HU9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1hu9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Glycine_max Glycine max]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HU9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HU9 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4HM:4-HYDROPEROXY-2-METHOXY-PHENOL'>4HM</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1lnh|1lnh]], [[1byt|1byt]], [[1yge|1yge]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4HM:4-HYDROPEROXY-2-METHOXY-PHENOL'>4HM</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Oxidoreductase Oxidoreductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.12 1.13.11.12] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hu9 OCA], [https://pdbe.org/1hu9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hu9 RCSB], [https://www.ebi.ac.uk/pdbsum/1hu9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hu9 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hu9 OCA], [http://pdbe.org/1hu9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1hu9 RCSB], [http://www.ebi.ac.uk/pdbsum/1hu9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1hu9 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/LOX3_SOYBN LOX3_SOYBN]] Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. It catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure. | + | [https://www.uniprot.org/uniprot/LOX3_SOYBN LOX3_SOYBN] Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. It catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Glycine max]] | | [[Category: Glycine max]] |
| - | [[Category: Oxidoreductase]] | + | [[Category: Large Structures]] |
| - | [[Category: Jankun, J]] | + | [[Category: Jankun J]] |
| - | [[Category: McCabe, N P]] | + | [[Category: McCabe NP]] |
| - | [[Category: Selman, S H]] | + | [[Category: Selman SH]] |
| - | [[Category: Skrzypczak-Jankun, E]] | + | [[Category: Skrzypczak-Jankun E]] |
| - | [[Category: Zhou, K]] | + | [[Category: Zhou K]] |
| - | [[Category: Enzyme inhibitor]]
| + | |
| - | [[Category: Metalloprotein]]
| + | |
| - | [[Category: Purple lipoxygenase]]
| + | |
| Structural highlights
Function
LOX3_SOYBN Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. It catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Scientific research provides documented evidence that fatty acid metabolites have profound impact on carcinogenesis. Intervention into dioxygenase pathways might therefore effect development, metastasis and progression of many types of cancers. This work delivers the first 3D structural data and explains how curcumin interacts with the fatty acid metabolizing enzyme, soybean lipoxygenase. Curcumin binds to lipoxygenase in a non-competitive manner. Trapped in that complex, it undergoes photodegradation in the X-rays, but utilizes enzyme catalytic ability to form the peroxy complex Enz-Fe-O-O-R as 4-hydroperoxy-2-methoxy-phenol, that later transforms into 2-methoxycyclohexa-2,5-diene-1,4-dione. Our observations about this radiation and time-dependent inhibition add new information to the role that curcumin might play in cancer prevention and treatment.
Structure of curcumin in complex with lipoxygenase and its significance in cancer.,Skrzypczak-Jankun E, Zhou K, McCabe NP, Selman SH, Jankun J Int J Mol Med. 2003 Jul;12(1):17-24. PMID:12792803[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Skrzypczak-Jankun E, Zhou K, McCabe NP, Selman SH, Jankun J. Structure of curcumin in complex with lipoxygenase and its significance in cancer. Int J Mol Med. 2003 Jul;12(1):17-24. PMID:12792803
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