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2gis

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[[Image:2gis.gif|left|200px]]
 
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{{Structure
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==Structure of the S-adenosylmethionine riboswitch mRNA regulatory element==
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|PDB= 2gis |SIZE=350|CAPTION= <scene name='initialview01'>2gis</scene>, resolution 2.90&Aring;
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<StructureSection load='2gis' size='340' side='right'caption='[[2gis]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=IRI:IRIDIUM+HEXAMMINE+ION'>IRI</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
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<table><tr><td colspan='2'>[[2gis]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus_subsp._tengcongensis Caldanaerobacter subterraneus subsp. tengcongensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GIS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GIS FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IRI:IRIDIUM+HEXAMMINE+ION'>IRI</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gis FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gis OCA], [https://pdbe.org/2gis PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gis RCSB], [https://www.ebi.ac.uk/pdbsum/2gis PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gis ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gis FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gis OCA], [http://www.ebi.ac.uk/pdbsum/2gis PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gis RCSB]</span>
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}}
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'''Structure of the S-adenosylmethionine riboswitch mRNA regulatory element'''
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==See Also==
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*[[Kink-turn motif|Kink-turn motif]]
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*[[Riboswitch 3D structures|Riboswitch 3D structures]]
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==Overview==
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*[[User:Wayne Decatur/kink-turn motif|User:Wayne Decatur/kink-turn motif]]
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Riboswitches are cis-acting genetic regulatory elements found in the 5'-untranslated regions of messenger RNAs that control gene expression through their ability to bind small molecule metabolites directly. Regulation occurs through the interplay of two domains of the RNA: an aptamer domain that responds to intracellular metabolite concentrations and an expression platform that uses two mutually exclusive secondary structures to direct a decision-making process. In Gram-positive bacteria such as Bacillus species, riboswitches control the expression of more than 2% of all genes through their ability to respond to a diverse set of metabolites including amino acids, nucleobases and protein cofactors. Here we report the 2.9-angstroms resolution crystal structure of an S-adenosylmethionine (SAM)-responsive riboswitch from Thermoanaerobacter tengcongensis complexed with S-adenosylmethionine, an RNA element that controls the expression of several genes involved in sulphur and methionine metabolism. This RNA folds into a complex three-dimensional architecture that recognizes almost every functional group of the ligand through a combination of direct and indirect readout mechanisms. Ligand binding induces the formation of a series of tertiary interactions with one of the helices, serving as a communication link between the aptamer and expression platform domains.
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Caldanaerobacter subterraneus subsp. tengcongensis]]
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2GIS is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GIS OCA].
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[[Category: Large Structures]]
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[[Category: Batey RT]]
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==Reference==
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[[Category: Montange RK]]
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Structure of the S-adenosylmethionine riboswitch regulatory mRNA element., Montange RK, Batey RT, Nature. 2006 Jun 29;441(7097):1172-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16810258 16810258]
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[[Category: Protein complex]]
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[[Category: Batey, R T.]]
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[[Category: Montange, R K.]]
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[[Category: mrna]]
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[[Category: riboswitch]]
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[[Category: rna-ligand complex]]
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[[Category: s-adenosylmethionine]]
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[[Category: sam]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:17:10 2008''
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Current revision

Structure of the S-adenosylmethionine riboswitch mRNA regulatory element

PDB ID 2gis

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