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1vqb
From Proteopedia
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==GENE V PROTEIN (SINGLE-STRANDED DNA BINDING PROTEIN)== | ==GENE V PROTEIN (SINGLE-STRANDED DNA BINDING PROTEIN)== | ||
| - | <StructureSection load='1vqb' size='340' side='right' caption='[[1vqb]], [[Resolution|resolution]] 1.80Å' scene=''> | + | <StructureSection load='1vqb' size='340' side='right'caption='[[1vqb]], [[Resolution|resolution]] 1.80Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[1vqb]] is a 1 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[1vqb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacteria_phage_f1 Enterobacteria phage f1]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1bgh 1bgh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VQB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VQB FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vqb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vqb OCA], [https://pdbe.org/1vqb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vqb RCSB], [https://www.ebi.ac.uk/pdbsum/1vqb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vqb ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | [ | + | [https://www.uniprot.org/uniprot/G5P_BPF1 G5P_BPF1] Binds to DNA in a highly cooperative manner without pronounced sequence specificity. During synthesis of the single-stranded (progeny) viral DNA, prevents the conversion into the double-stranded replicative form. G5P is displaced by the capsid protein G8P during phage assembly on the inner bacterial membrane (By similarity). |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1vqb ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1vqb ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | The crystal structure of the dimeric gene V protein of bacteriophage f1 was determined using multiwavelength anomalous diffraction on the selenomethionine-containing wild-type and isoleucine-47-->methionine mutant proteins with x-ray diffraction data phased to 2.5 A resolution. The structure of the wild-type protein has been refined to an R factor of 19.2% using native data to 1.8 A resolution. The structure of the gene V protein was used to obtain a model for the protein portion of the gene V protein-single-stranded DNA complex. | ||
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| - | Structure of the gene V protein of bacteriophage f1 determined by multiwavelength x-ray diffraction on the selenomethionyl protein.,Skinner MM, Zhang H, Leschnitzer DH, Guan Y, Bellamy H, Sweet RM, Gray CW, Konings RN, Wang AH, Terwilliger TC Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2071-5. PMID:8134350<ref>PMID:8134350</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 1vqb" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
| - | *[[Single-stranded DNA-binding protein|Single-stranded DNA-binding protein]] | + | *[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]] |
| - | + | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Enterobacteria phage f1]] |
| - | [[Category: Bellamy | + | [[Category: Large Structures]] |
| - | [[Category: Gray | + | [[Category: Bellamy H]] |
| - | [[Category: Guan | + | [[Category: Gray CW]] |
| - | [[Category: Konings | + | [[Category: Guan Y]] |
| - | [[Category: Leschnitzer | + | [[Category: Konings RNH]] |
| - | [[Category: Skinner | + | [[Category: Leschnitzer DH]] |
| - | [[Category: Sweet | + | [[Category: Skinner MM]] |
| - | [[Category: Terwilliger | + | [[Category: Sweet RM]] |
| - | [[Category: Wang | + | [[Category: Terwilliger TC]] |
| - | [[Category: Zhang | + | [[Category: Wang AH-J]] |
| - | + | [[Category: Zhang H]] | |
| - | + | ||
Current revision
GENE V PROTEIN (SINGLE-STRANDED DNA BINDING PROTEIN)
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