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| | ==FERREDOXIN-NADP+ REDUCTASE (MUTATION: Y 303 W)== | | ==FERREDOXIN-NADP+ REDUCTASE (MUTATION: Y 303 W)== |
| - | <StructureSection load='1w35' size='340' side='right' caption='[[1w35]], [[Resolution|resolution]] 1.90Å' scene=''> | + | <StructureSection load='1w35' size='340' side='right'caption='[[1w35]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1w35]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Anabaena_pcc7119 Anabaena pcc7119]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W35 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1W35 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1w35]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_sp._PCC_7119 Nostoc sp. PCC 7119]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W35 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1W35 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1b2r|1b2r]], [[1bjk|1bjk]], [[1bqe|1bqe]], [[1e62|1e62]], [[1e63|1e63]], [[1e64|1e64]], [[1ewy|1ewy]], [[1gjr|1gjr]], [[1go2|1go2]], [[1gr1|1gr1]], [[1h42|1h42]], [[1h85|1h85]], [[1ogi|1ogi]], [[1ogj|1ogj]], [[1qgy|1qgy]], [[1qgz|1qgz]], [[1qh0|1qh0]], [[1que|1que]], [[1quf|1quf]], [[1w34|1w34]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferredoxin--NADP(+)_reductase Ferredoxin--NADP(+) reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.1.2 1.18.1.2] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1w35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w35 OCA], [https://pdbe.org/1w35 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1w35 RCSB], [https://www.ebi.ac.uk/pdbsum/1w35 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1w35 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w35 OCA], [http://pdbe.org/1w35 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1w35 RCSB], [http://www.ebi.ac.uk/pdbsum/1w35 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1w35 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/FENR_NOSSO FENR_NOSSO] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Anabaena pcc7119]] | + | [[Category: Large Structures]] |
| - | [[Category: Gomez-Moreno, C]] | + | [[Category: Nostoc sp. PCC 7119]] |
| - | [[Category: Hermoso, J A]] | + | [[Category: Gomez-Moreno C]] |
| - | [[Category: Julvez, M M]] | + | [[Category: Hermoso JA]] |
| - | [[Category: Medina, M]] | + | [[Category: Julvez MM]] |
| - | [[Category: Perez-Dorado, I]] | + | [[Category: Medina M]] |
| - | [[Category: Sanz-Aparicio, J]] | + | [[Category: Perez-Dorado I]] |
| - | [[Category: Fad]]
| + | [[Category: Sanz-Aparicio J]] |
| - | [[Category: Flavoprotein]]
| + | |
| - | [[Category: Fnr]]
| + | |
| - | [[Category: Nadp]]
| + | |
| - | [[Category: Nadp reductase]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| Structural highlights
Function
FENR_NOSSO
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Ferredoxin-NADP+ reductase (FNR) catalyzes the reduction of NADP+ to NADPH in an overall reversible reaction, showing some differences in the mechanisms between cyanobacterial and higher plant FNRs. During hydride transfer it is proposed that the FNR C-terminal Tyr is displaced by the nicotinamide. Thus, this C-terminal Tyr might be involved not only in modulating the flavin redox properties, as already shown, but also in nicotinamide binding and hydride transfer. FNR variants from the cyanobacterium Anabaena in which the C-terminal Tyr has been replaced by Trp, Phe, or Ser have been produced. All FNR variants show enhanced NADP+ and NAD+ binding, especially Tyr303Ser, which correlates with a noticeable improvement of NADH-dependent reactions. Nevertheless, the Tyr303Ser variant shows a decrease in the steady-state kcat value with NADPH. Fast kinetic analysis of the hydride transfer shows that the low efficiency observed for this mutant FNR under steady-state conditions is not due to a lack of catalytic ability but rather to the strong enzyme-coenzyme interaction. Three-dimensional structures for Tyr303Ser and Tyr303Trp variants and its complexes with NADP+ show significant differences between plant and cyanobacterial FNRs. Our results suggest that modulation of coenzyme affinity is highly influenced by the strength of the C-terminus-FAD interaction and that subtle changes between plant and cyanobacterial structures are able to modify the energy of that interaction. Additionally, it is shown that the C-terminal Tyr of FNR lowers the affinity for NADP+/H to levels compatible with steady-state turnover during the catalytic cycle, but it is not involved in the hydride transfer itself.
C-terminal tyrosine of ferredoxin-NADP+ reductase in hydride transfer processes with NAD(P)+/H.,Tejero J, Perez-Dorado I, Maya C, Martinez-Julvez M, Sanz-Aparicio J, Gomez-Moreno C, Hermoso JA, Medina M Biochemistry. 2005 Oct 18;44(41):13477-90. PMID:16216071[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Tejero J, Perez-Dorado I, Maya C, Martinez-Julvez M, Sanz-Aparicio J, Gomez-Moreno C, Hermoso JA, Medina M. C-terminal tyrosine of ferredoxin-NADP+ reductase in hydride transfer processes with NAD(P)+/H. Biochemistry. 2005 Oct 18;44(41):13477-90. PMID:16216071 doi:10.1021/bi051278c
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