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| | ==X-ray structure of peptidyl-prolyl cis-trans isomerase A, PpiA, Rv0009, from Mycobacterium tuberculosis.== | | ==X-ray structure of peptidyl-prolyl cis-trans isomerase A, PpiA, Rv0009, from Mycobacterium tuberculosis.== |
| - | <StructureSection load='1w74' size='340' side='right' caption='[[1w74]], [[Resolution|resolution]] 2.60Å' scene=''> | + | <StructureSection load='1w74' size='340' side='right'caption='[[1w74]], [[Resolution|resolution]] 2.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1w74]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W74 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1W74 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1w74]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W74 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1W74 FirstGlance]. <br> |
| - | </td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptidylprolyl_isomerase Peptidylprolyl isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.2.1.8 5.2.1.8] </span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w74 OCA], [http://pdbe.org/1w74 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1w74 RCSB], [http://www.ebi.ac.uk/pdbsum/1w74 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1w74 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1w74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w74 OCA], [https://pdbe.org/1w74 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1w74 RCSB], [https://www.ebi.ac.uk/pdbsum/1w74 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1w74 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/PPIA_MYCTU PPIA_MYCTU] PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.<ref>PMID:15479239</ref> <ref>PMID:17141764</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| - | *[[Cyclophilin|Cyclophilin]] | + | *[[Cyclophilin 3D structures|Cyclophilin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Myctu]] | + | [[Category: Large Structures]] |
| - | [[Category: Peptidylprolyl isomerase]] | + | [[Category: Mycobacterium tuberculosis H37Rv]] |
| - | [[Category: Henriksson, L M]] | + | [[Category: Henriksson LM]] |
| - | [[Category: Johansson, P]] | + | [[Category: Johansson P]] |
| - | [[Category: Mowbray, S L]] | + | [[Category: Mowbray SL]] |
| - | [[Category: Unge, T]] | + | [[Category: Unge T]] |
| - | [[Category: Cyclophilin]]
| + | |
| - | [[Category: Isomerase]]
| + | |
| - | [[Category: Peptidyl-prolyl cis-trans isomerase]]
| + | |
| - | [[Category: Ppiase]]
| + | |
| - | [[Category: Rotamase]]
| + | |
| - | [[Category: Rv0009]]
| + | |
| - | [[Category: Spine]]
| + | |
| - | [[Category: Structural genomic]]
| + | |
| - | [[Category: Structural proteomics in europe]]
| + | |
| Structural highlights
Function
PPIA_MYCTU PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Peptidyl-prolyl cis-trans isomerases (EC 5.2.1.8) catalyse the interconversion of cis and trans peptide bonds and are therefore considered to be important for protein folding. They are also thought to participate in processes such as signalling, cell surface recognition, chaperoning and heat-shock response. Here we report the soluble expression of recombinant Mycobacterium tuberculosis peptidyl-prolyl cis-trans isomerase PpiA in Escherichia coli, together with an investigation of its structure and biochemical properties. The protein was shown to be active in a spectrophotometric assay, with an estimated kcat/Km of 2.0 x 10(6) m(-1).s(-1). The X-ray structure of PpiA was solved by molecular replacement, and refined to a resolution of 2.6 A with R and Rfree values of 21.3% and 22.9%, respectively. Comparisons to known structures show that the PpiA represents a slight variation on the peptidyl-prolyl cis-trans isomerase fold, previously not represented in the Protein Data Bank. Inspection of the active site suggests that specificity for substrates and cyclosporin A will be similar to that found for most other enzymes of this structural family. Comparison to the sequence of the second M. tuberculosis enzyme, PpiB, suggests that binding of peptide substrates as well as cyclosporin A may differ in that case.
X-ray structure of peptidyl-prolyl cis-trans isomerase A from Mycobacterium tuberculosis.,Henriksson LM, Johansson P, Unge T, Mowbray SL Eur J Biochem. 2004 Oct;271(20):4107-13. PMID:15479239[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Henriksson LM, Johansson P, Unge T, Mowbray SL. X-ray structure of peptidyl-prolyl cis-trans isomerase A from Mycobacterium tuberculosis. Eur J Biochem. 2004 Oct;271(20):4107-13. PMID:15479239 doi:10.1111/j.1432-1033.2004.04348.x
- ↑ Mitra D, Mukherjee S, Das AK. Cyclosporin A binding to Mycobacterium tuberculosis peptidyl-prolyl cis-trans isomerase A--investigation by CD, FTIR and fluorescence spectroscopy. FEBS Lett. 2006 Dec 22;580(30):6846-60. PMID:17141764 doi:10.1016/j.febslet.2006.11.042
- ↑ Henriksson LM, Johansson P, Unge T, Mowbray SL. X-ray structure of peptidyl-prolyl cis-trans isomerase A from Mycobacterium tuberculosis. Eur J Biochem. 2004 Oct;271(20):4107-13. PMID:15479239 doi:10.1111/j.1432-1033.2004.04348.x
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