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2grb

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[[Image:2grb.gif|left|200px]]
 
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{{Structure
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==Crystal Structure of an RNA Quadruplex Containing Inosine-tetrad==
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|PDB= 2grb |SIZE=350|CAPTION= <scene name='initialview01'>2grb</scene>, resolution 1.40&Aring;
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<StructureSection load='2grb' size='340' side='right'caption='[[2grb]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=I:INOSINIC+ACID'>I</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>, <scene name='pdbligand=U33:5-BROMO-2&#39;-DEOXY+URIDINE'>U33</scene>
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<table><tr><td colspan='2'>[[2grb]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GRB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GRB FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene>, <scene name='pdbligand=U33:5-BROMO-2-DEOXY+URIDINE'>U33</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2grb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2grb OCA], [https://pdbe.org/2grb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2grb RCSB], [https://www.ebi.ac.uk/pdbsum/2grb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2grb ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2grb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2grb OCA], [http://www.ebi.ac.uk/pdbsum/2grb PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2grb RCSB]</span>
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__TOC__
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}}
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</StructureSection>
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[[Category: Large Structures]]
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'''Crystal Structure of an RNA Quadruplex Containing Inosine-tetrad'''
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[[Category: Pan B]]
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[[Category: Shi K]]
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[[Category: Sundaralingam M]]
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==Overview==
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Polyinosinic acid has been known to adopt the four-stranded helical structure but its basic unit, inosine tetrad (I tetrad), has not been determined at the atomic level. Here we report the crystal structure of an RNA quadruplex containing an I tetrad at 1.4 A resolution. The I tetrad has one cyclic hydrogen bond N1...O6 with the bond length of 2.7 A. A water bridge is observed in the minor groove side of the base tetrad. Even though it is sandwiched by guanine tetrads (G tetrads), the I tetrad is buckled towards the 3' side of the tetrad plane, which results from the different interaction strength with K ions on two sides of the tetrad plane. Comparison with both G tetrad and adenine tetrad indicates that lack of NH2 in the C2 position makes the I tetrad prone to buckle for interactions with ligands. Two U*(G-G-G-G) base pentads are observed at the junction of the 5' termini of two quadruplexes. The uridine residue in the base pentad is engaged in two hydrogen bonding interactions (N2(G)-H...O2(U) and O2'(G)-H...O4(U)) and a water-mediated interaction (N3(G) and N3(U)) with the G tetrad. We also discuss the roles of amino group in purine tetrads and the inter-quadruplex interactions in RNA molecules. These quadruplexes may interact with each other by stacking, groove binding and intercalation.
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==About this Structure==
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2GRB is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GRB OCA].
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==Reference==
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Crystal structure of an RNA quadruplex containing inosine tetrad: implications for the roles of NH2 group in purine tetrads., Pan B, Shi K, Sundaralingam M, J Mol Biol. 2006 Oct 20;363(2):451-9. Epub 2006 Aug 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16978642 16978642]
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[[Category: Protein complex]]
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[[Category: Pan, B.]]
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[[Category: Shi, K.]]
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[[Category: Sundaralingam, M.]]
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[[Category: rna quadruplex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:20:29 2008''
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Current revision

Crystal Structure of an RNA Quadruplex Containing Inosine-tetrad

PDB ID 2grb

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