2gr7

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[[Image:2gr7.gif|left|200px]]
 
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{{Structure
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==Hia 992-1098==
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|PDB= 2gr7 |SIZE=350|CAPTION= <scene name='initialview01'>2gr7</scene>, resolution 2.300&Aring;
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<StructureSection load='2gr7' size='340' side='right'caption='[[2gr7]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>
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<table><tr><td colspan='2'>[[2gr7]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GR7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GR7 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gr7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gr7 OCA], [https://pdbe.org/2gr7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gr7 RCSB], [https://www.ebi.ac.uk/pdbsum/2gr7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gr7 ProSAT]</span></td></tr>
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|RELATEDENTRY=[[2gr8|2GR8]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gr7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gr7 OCA], [http://www.ebi.ac.uk/pdbsum/2gr7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gr7 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/Q48152_HAEIF Q48152_HAEIF]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gr/2gr7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gr7 ConSurf].
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<div style="clear:both"></div>
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'''Hia 992-1098'''
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==See Also==
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*[[Adhesin 3D structures|Adhesin 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Autotransporter proteins are defined by the ability to drive their own secretion across the bacterial outer membrane. The Hia autotransporter of Haemophilus influenzae belongs to the trimeric autotransporter subfamily and mediates bacterial adhesion to the respiratory epithelium. In this report, we present the crystal structure of the C-terminal end of Hia, corresponding to the entire Hia translocator domain and part of the passenger domain (residues 992-1098). This domain forms a beta-barrel with 12 transmembrane beta-strands, including four strands from each subunit. The beta-barrel has a central channel of 1.8 nm in diameter that is traversed by three N-terminal alpha-helices, one from each subunit. Mutagenesis studies demonstrate that the transmembrane portion of the three alpha-helices and the loop region between the alpha-helices and the neighboring beta-strands are essential for stability of the trimeric structure of the translocator domain, and that trimerization of the translocator domain is a prerequisite for translocator activity. Overall, this study provides important insights into the mechanism of translocation in trimeric autotransporters.
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==About this Structure==
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2GR7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GR7 OCA].
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==Reference==
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Structure of the outer membrane translocator domain of the Haemophilus influenzae Hia trimeric autotransporter., Meng G, Surana NK, St Geme JW 3rd, Waksman G, EMBO J. 2006 Jun 7;25(11):2297-304. Epub 2006 May 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16688217 16688217]
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[[Category: Haemophilus influenzae]]
[[Category: Haemophilus influenzae]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Meng, G.]]
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[[Category: Meng G]]
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[[Category: Waksman, G.]]
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[[Category: Waksman G]]
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[[Category: adhesion]]
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[[Category: crystal structure]]
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[[Category: membrane protein]]
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[[Category: microbial pathogenesis]]
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[[Category: protein secretion]]
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[[Category: trimeric autotransporter]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:20:24 2008''
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Current revision

Hia 992-1098

PDB ID 2gr7

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