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2grf

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[[Image:2grf.gif|left|200px]]
 
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{{Structure
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==Crystal structure of Scapharca inaequivalvis HBI, M37V mutant in the absence of ligand==
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|PDB= 2grf |SIZE=350|CAPTION= <scene name='initialview01'>2grf</scene>, resolution 2.10&Aring;
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<StructureSection load='2grf' size='340' side='right'caption='[[2grf]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>
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<table><tr><td colspan='2'>[[2grf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Anadara_inaequivalvis Anadara inaequivalvis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GRF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GRF FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2grf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2grf OCA], [https://pdbe.org/2grf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2grf RCSB], [https://www.ebi.ac.uk/pdbsum/2grf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2grf ProSAT]</span></td></tr>
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|RELATEDENTRY=[[4sdh|4SDH]], [[2grh|2GRH]], [[2grz|2GRZ]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2grf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2grf OCA], [http://www.ebi.ac.uk/pdbsum/2grf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2grf RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/GLB1_ANAIN GLB1_ANAIN]
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== Evolutionary Conservation ==
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'''Crystal structure of Scapharca inaequivalvis HBI, M37V mutant in the absence of ligand'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gr/2grf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2grf ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
Protein allostery provides mechanisms for regulation of biological function at the molecular level. We present here an investigation of global, ligand-induced allosteric transition in a protein by time-resolved x-ray diffraction. The study provides a view of structural changes in single crystals of Scapharca dimeric hemoglobin as they proceed in real time, from 5 ns to 80 micros after ligand photodissociation. A tertiary intermediate structure forms rapidly (&lt;5 ns) as the protein responds to the presence of an unliganded heme within each R-state protein subunit, with key structural changes observed in the heme groups, neighboring residues, and interface water molecules. This intermediate lays a foundation for the concerted tertiary and quaternary structural changes that occur on a microsecond time scale and are associated with the transition to a low-affinity T-state structure. Reversal of these changes shows a considerable lag as a T-like structure persists well after ligand rebinding, suggesting a slow T-to-R transition.
Protein allostery provides mechanisms for regulation of biological function at the molecular level. We present here an investigation of global, ligand-induced allosteric transition in a protein by time-resolved x-ray diffraction. The study provides a view of structural changes in single crystals of Scapharca dimeric hemoglobin as they proceed in real time, from 5 ns to 80 micros after ligand photodissociation. A tertiary intermediate structure forms rapidly (&lt;5 ns) as the protein responds to the presence of an unliganded heme within each R-state protein subunit, with key structural changes observed in the heme groups, neighboring residues, and interface water molecules. This intermediate lays a foundation for the concerted tertiary and quaternary structural changes that occur on a microsecond time scale and are associated with the transition to a low-affinity T-state structure. Reversal of these changes shows a considerable lag as a T-like structure persists well after ligand rebinding, suggesting a slow T-to-R transition.
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==About this Structure==
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Allosteric action in real time: time-resolved crystallographic studies of a cooperative dimeric hemoglobin.,Knapp JE, Pahl R, Srajer V, Royer WE Jr Proc Natl Acad Sci U S A. 2006 May 16;103(20):7649-54. Epub 2006 May 9. PMID:16684887<ref>PMID:16684887</ref>
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2GRF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Scapharca_inaequivalvis Scapharca inaequivalvis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GRF OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Allosteric action in real time: time-resolved crystallographic studies of a cooperative dimeric hemoglobin., Knapp JE, Pahl R, Srajer V, Royer WE Jr, Proc Natl Acad Sci U S A. 2006 May 16;103(20):7649-54. Epub 2006 May 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16684887 16684887]
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</div>
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[[Category: Scapharca inaequivalvis]]
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<div class="pdbe-citations 2grf" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Jr., W E.Royer.]]
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[[Category: Knapp, J E.]]
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[[Category: Pahl, R.]]
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[[Category: Srajer, V.]]
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[[Category: allostery]]
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[[Category: cooperativity]]
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[[Category: heme protein]]
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[[Category: hemoglobin]]
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[[Category: invertebrate]]
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[[Category: oxygen-binding]]
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[[Category: oxygen-transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:20:29 2008''
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==See Also==
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*[[Hemoglobin 3D structures|Hemoglobin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Anadara inaequivalvis]]
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[[Category: Large Structures]]
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[[Category: Knapp JE]]
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[[Category: Pahl R]]
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[[Category: Royer Jr WE]]
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[[Category: Srajer V]]

Current revision

Crystal structure of Scapharca inaequivalvis HBI, M37V mutant in the absence of ligand

PDB ID 2grf

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