|
|
| (One intermediate revision not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Trans-acting transferase from Disorazole synthase solved by serial femtosecond XFEL crystallography== | | ==Trans-acting transferase from Disorazole synthase solved by serial femtosecond XFEL crystallography== |
| - | <StructureSection load='6apk' size='340' side='right' caption='[[6apk]], [[Resolution|resolution]] 2.50Å' scene=''> | + | <StructureSection load='6apk' size='340' side='right'caption='[[6apk]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6apk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"polyangium_compositum"_thaxter_1904 "polyangium compositum" thaxter 1904]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6APK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6APK FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6apk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sorangium_cellulosum Sorangium cellulosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6APK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6APK FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dszD, disD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=56 "Polyangium compositum" Thaxter 1904])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/[Acyl-carrier-protein]_S-malonyltransferase [Acyl-carrier-protein] S-malonyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.39 2.3.1.39] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6apk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6apk OCA], [https://pdbe.org/6apk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6apk RCSB], [https://www.ebi.ac.uk/pdbsum/6apk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6apk ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6apk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6apk OCA], [http://pdbe.org/6apk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6apk RCSB], [http://www.ebi.ac.uk/pdbsum/6apk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6apk ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q4U443_SORCE Q4U443_SORCE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 21: |
Line 22: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Polyangium compositum thaxter 1904]] | + | [[Category: Large Structures]] |
| - | [[Category: Cohen, A E]] | + | [[Category: Sorangium cellulosum]] |
| - | [[Category: Khosla, C]] | + | [[Category: Cohen AE]] |
| - | [[Category: Lyubimov, A Y]] | + | [[Category: Khosla C]] |
| - | [[Category: Mathews, I I]] | + | [[Category: Lyubimov AY]] |
| - | [[Category: Soltis, S M]] | + | [[Category: Mathews II]] |
| - | [[Category: Uervivojnangkoorn, M]] | + | [[Category: Soltis SM]] |
| - | [[Category: Disorazole synthase]]
| + | [[Category: Uervivojnangkoorn M]] |
| - | [[Category: Serial femtosecond crystallography]]
| + | |
| - | [[Category: Transferase]]
| + | |
| - | [[Category: Xfel crystal structure]]
| + | |
| Structural highlights
Function
Q4U443_SORCE
Publication Abstract from PubMed
The crystal structure of the trans-acyltransferase (AT) from the disorazole polyketide synthase (PKS) was determined at room temperature to a resolution of 2.5 A using a new method for the direct delivery of the sample into an X-ray free-electron laser. A novel sample extractor efficiently delivered limited quantities of microcrystals directly from the native crystallization solution into the X-ray beam at room temperature. The AT structure revealed important catalytic features of this core PKS enzyme, including the occurrence of conformational changes around the active site. The implications of these conformational changes for polyketide synthase reaction dynamics are discussed.
The Conformational Flexibility of the Acyltransferase from the Disorazole Polyketide Synthase Is Revealed by an X-ray Free-Electron Laser Using a Room-Temperature Sample Delivery Method for Serial Crystallography.,Mathews II, Allison K, Robbins T, Lyubimov AY, Uervirojnangkoorn M, Brunger AT, Khosla C, DeMirci H, McPhillips SE, Hollenbeck M, Soltis M, Cohen AE Biochemistry. 2017 Sep 12;56(36):4751-4756. doi: 10.1021/acs.biochem.7b00711., Epub 2017 Aug 31. PMID:28832129[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Mathews II, Allison K, Robbins T, Lyubimov AY, Uervirojnangkoorn M, Brunger AT, Khosla C, DeMirci H, McPhillips SE, Hollenbeck M, Soltis M, Cohen AE. The Conformational Flexibility of the Acyltransferase from the Disorazole Polyketide Synthase Is Revealed by an X-ray Free-Electron Laser Using a Room-Temperature Sample Delivery Method for Serial Crystallography. Biochemistry. 2017 Sep 12;56(36):4751-4756. doi: 10.1021/acs.biochem.7b00711., Epub 2017 Aug 31. PMID:28832129 doi:http://dx.doi.org/10.1021/acs.biochem.7b00711
|
