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| | ==Crystal Structure of Multimeric DNA-binding Protein Sac7d-GCN4 with DNA decamer== | | ==Crystal Structure of Multimeric DNA-binding Protein Sac7d-GCN4 with DNA decamer== |
| - | <StructureSection load='1wvl' size='340' side='right' caption='[[1wvl]], [[Resolution|resolution]] 2.60Å' scene=''> | + | <StructureSection load='1wvl' size='340' side='right'caption='[[1wvl]], [[Resolution|resolution]] 2.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1wvl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33909 Atcc 33909]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WVL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WVL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1wvl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WVL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WVL FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1azp|1azp]], [[1wd0|1wd0]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wvl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wvl OCA], [http://pdbe.org/1wvl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wvl RCSB], [http://www.ebi.ac.uk/pdbsum/1wvl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1wvl ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wvl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wvl OCA], [https://pdbe.org/1wvl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wvl RCSB], [https://www.ebi.ac.uk/pdbsum/1wvl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wvl ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/DN71_SULAC DN71_SULAC]] Constrain negative DNA supercoils; may be involved in maintaining the integrity of their genome at high temperature. | + | [https://www.uniprot.org/uniprot/DN7D_SULAC DN7D_SULAC] Can constrain negative DNA supercoils. May be involved in maintaining the integrity of the genome at high temperature.[UniProtKB:P61990] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Gcn4|Gcn4]] | + | *[[Gcn4 3D Structures|Gcn4 3D Structures]] |
| | + | *[[Gnc4 3D Structures|Gnc4 3D Structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 33909]] | + | [[Category: Large Structures]] |
| - | [[Category: Wang, A H]] | + | [[Category: Sulfolobus acidocaldarius]] |
| - | [[Category: Wu, S W]] | + | [[Category: Wang AH]] |
| - | [[Category: Leucine zipper]] | + | [[Category: Wu SW]] |
| - | [[Category: Protein engineering]]
| + | |
| - | [[Category: Structural protein-dna complex]]
| + | |
| - | [[Category: Synchrotron radiation]]
| + | |
| - | [[Category: Thermophile]]
| + | |
| Structural highlights
Function
DN7D_SULAC Can constrain negative DNA supercoils. May be involved in maintaining the integrity of the genome at high temperature.[UniProtKB:P61990]
Publication Abstract from PubMed
The protein Sac7d belongs to a class of small chromosomal proteins from the hyperthermophilic archaeon Sulfolobus acidocaldarius. Sac7d is extremely stable to heat, acid, and chemical agents. This protein is a monomer and it binds DNA without any particular sequence preference, while inducing a sharp kink in the DNA. By appending a leucine-zipper-like helical peptide derived from the yeast transcriptional activator GCN4 to the C-terminal end of Sac7d, the modified monomers (denoted S7dLZ) are expected to interact with each other via hydrophobic force to form a parallel dimer. The recombinant S7dLZ was expressed in Escherichia coli and purified by heating and ion-exchange chromatography. The formation of dimer was detected by gel-filtration chromatography and chemical cross-link. The results of surface plasmon resonance and circular dichroism experiments showed that the DNA-binding capacity was retained. Furthermore, X-ray diffraction analysis of single crystals of S7dLZ in complex with DNA decamer CCTATATAGG showed that the leucine-zipper segments of S7dLZ were associated into an antiparallel four-helix bundle. There are two DNA fragments bound to each S7dLZ tetramer in the crystal. This model works as a successful template that endows protein a new function without losing original properties.
Design and characterization of a multimeric DNA binding protein using Sac7d and GCN4 as templates.,Wu SW, Ko TP, Chou CC, Wang AH Proteins. 2005 Sep 1;60(4):617-28. PMID:16028219[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Wu SW, Ko TP, Chou CC, Wang AH. Design and characterization of a multimeric DNA binding protein using Sac7d and GCN4 as templates. Proteins. 2005 Sep 1;60(4):617-28. PMID:16028219 doi:http://dx.doi.org/10.1002/prot.20524
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