|
|
| (11 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| - | [[Image:2h1m.gif|left|200px]] | |
| | | | |
| - | {{Structure
| + | ==Synthesis, Oxidation Behavior, Crystallization and Structure of 2'-Methylseleno Guanosine Containing RNAs== |
| - | |PDB= 2h1m |SIZE=350|CAPTION= <scene name='initialview01'>2h1m</scene>, resolution 2.90Å
| + | <StructureSection load='2h1m' size='340' side='right'caption='[[2h1m]], [[Resolution|resolution]] 2.90Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene>, <scene name='pdbligand=XUG:2'-SE-METHYL-2'-SELENOGUANOSINE+5'-(DIHYDROGEN+PHOSPHATE)'>XUG</scene>
| + | <table><tr><td colspan='2'>[[2h1m]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H1M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H1M FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XUG:2-SE-METHYL-2-SELENOGUANOSINE+5-(DIHYDROGEN+PHOSPHATE)'>XUG</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h1m OCA], [https://pdbe.org/2h1m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h1m RCSB], [https://www.ebi.ac.uk/pdbsum/2h1m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h1m ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h1m OCA], [http://www.ebi.ac.uk/pdbsum/2h1m PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2h1m RCSB]</span>
| + | __TOC__ |
| - | }}
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | '''Synthesis, Oxidation Behavior, Crystallization and Structure of 2'-Methylseleno Guanosine Containing RNAs'''
| + | [[Category: Serganov AA]] |
| - | | + | |
| - | | + | |
| - | ==Overview==
| + | |
| - | We have recently introduced a basic concept for the combined chemical and enzymatic preparation of site-specifically modified 2'-methylseleno RNAs which represent useful derivatives for phasing of X-ray crystallographic data during their three-dimensional structure determination. Here, we introduce the first synthesis of an appropriate guanosine phosphoramidite, which complements the thus far established set of 2'-methylseleno-modified uridine, cytidine, and adenosine building blocks for solid-phase synthesis. The novel building block was readily incorporated into RNA. Importantly, it was the 2'-methylseleno-guanosine-labeled RNA that allowed us to reveal the reversible oxidation/reduction behavior of the Se moiety and thus it represents a valuable contribution to the understanding of the action of threo-1,4-dimercapto-2,3-butanediol (DTT) required during solid-phase synthesis, deprotection, and crystallization of selenium-containing RNA. In addition, we investigated 2'-methylseleno RNA with respect to crystallization properties. Our studies revealed that the Se modification significantly increases the range of conditions leading to crystal growth. Moreover, we determined the crystal structures of model RNA helices and showed that the Se modification can affect crystal packing interactions, thus potentially expanding the possibilities for obtaining the best crystal form.
| + | |
| - | | + | |
| - | ==About this Structure==
| + | |
| - | 2H1M is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H1M OCA].
| + | |
| - | | + | |
| - | ==Reference==
| + | |
| - | Synthesis, oxidation behavior, crystallization and structure of 2'-methylseleno guanosine containing RNAs., Moroder H, Kreutz C, Lang K, Serganov A, Micura R, J Am Chem Soc. 2006 Aug 2;128(30):9909-18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16866550 16866550]
| + | |
| - | [[Category: Protein complex]]
| + | |
| - | [[Category: Serganov, A A.]]
| + | |
| - | [[Category: a-rna structure]] | + | |
| - | [[Category: mismatch a rna]] | + | |
| - | [[Category: ribonucleic acid]]
| + | |
| - | [[Category: se 2'-methylse]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:24:14 2008''
| + | |
