4ru4

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==Crystal structure of the tailspike protein gp49 from Pseudomonas phage LKA1==
==Crystal structure of the tailspike protein gp49 from Pseudomonas phage LKA1==
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<StructureSection load='4ru4' size='340' side='right' caption='[[4ru4]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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<StructureSection load='4ru4' size='340' side='right'caption='[[4ru4]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4ru4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_lka1 Bacteriophage lka1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RU4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RU4 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4ru4]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_phage_LKA1 Pseudomonas phage LKA1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RU4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RU4 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.903&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gp49, PPLKA1_gp49 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=386793 Bacteriophage LKA1])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ru4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ru4 OCA], [http://pdbe.org/4ru4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ru4 RCSB], [http://www.ebi.ac.uk/pdbsum/4ru4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ru4 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ru4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ru4 OCA], [https://pdbe.org/4ru4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ru4 RCSB], [https://www.ebi.ac.uk/pdbsum/4ru4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ru4 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q0E5W5_9CAUD Q0E5W5_9CAUD]
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Pseudomonas phage LKA1 of the subfamily Autographivirinae encodes a tailspike protein (LKA1gp49) which binds and cleaves B-band LPS (O-specific antigen, OSA) of Pseudomonas aeruginosa PAO1. The crystal structure of LKA1gp49 catalytic domain consists of a beta-helix, an insertion domain and a C-terminal discoidin-like domain. The putative substrate binding and processing site is located on the face of the beta-helix whereas the C-terminal domain is likely involved in carbohydrates binding. NMR spectroscopy and mass spectrometry analyses of degraded LPS (OSA) fragments show an O5 serotype-specific polysaccharide lyase specificity. LKA1gp49 reduces virulence in an in vivo Galleria mellonella infection model and sensitizes P. aeruginosa to serum complement activity. This enzyme causes biofilm degradation and does not affect the activity of ciprofloxacin and gentamicin. This is the first comprehensive report on LPS-degrading lyase derived from a Pseudomonas phage. Biological properties reveal a potential towards its applications in antimicrobial design and as a microbiological or biotechnological tool.
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The O-specific polysaccharide lyase from the phage LKA1 tailspike reduces Pseudomonas virulence.,Olszak T, Shneider MM, Latka A, Maciejewska B, Browning C, Sycheva LV, Cornelissen A, Danis-Wlodarczyk K, Senchenkova SN, Shashkov AS, Gula G, Arabski M, Wasik S, Miroshnikov KA, Lavigne R, Leiman PG, Knirel YA, Drulis-Kawa Z Sci Rep. 2017 Nov 24;7(1):16302. doi: 10.1038/s41598-017-16411-4. PMID:29176754<ref>PMID:29176754</ref>
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==See Also==
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*[[Tailspike protein 3D structures|Tailspike protein 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4ru4" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bacteriophage lka1]]
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[[Category: Large Structures]]
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[[Category: Browning, C]]
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[[Category: Pseudomonas phage LKA1]]
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[[Category: Leiman, P G]]
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[[Category: Browning C]]
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[[Category: Shneider, M M]]
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[[Category: Leiman PG]]
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[[Category: Baseplate]]
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[[Category: Shneider MM]]
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[[Category: Lyase]]
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[[Category: Phage lka1]]
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[[Category: Structural protein]]
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[[Category: Tail spike protein]]
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Current revision

Crystal structure of the tailspike protein gp49 from Pseudomonas phage LKA1

PDB ID 4ru4

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