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| | ==Structure of the C-terminal domains of DipZ from Mycobacterium tuberculosis== | | ==Structure of the C-terminal domains of DipZ from Mycobacterium tuberculosis== |
| - | <StructureSection load='5cyy' size='340' side='right' caption='[[5cyy]], [[Resolution|resolution]] 2.20Å' scene=''> | + | <StructureSection load='5cyy' size='340' side='right'caption='[[5cyy]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5cyy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CYY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CYY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5cyy]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CYY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CYY FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2hyx|2hyx]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cyy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cyy OCA], [https://pdbe.org/5cyy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cyy RCSB], [https://www.ebi.ac.uk/pdbsum/5cyy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cyy ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dipZ, Rv2874, MTCY274.05 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cyy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cyy OCA], [http://pdbe.org/5cyy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cyy RCSB], [http://www.ebi.ac.uk/pdbsum/5cyy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5cyy ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/DIPZ_MYCTU DIPZ_MYCTU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Myctu]] | + | [[Category: Large Structures]] |
| - | [[Category: Baker, E N]]
| + | [[Category: Mycobacterium tuberculosis H37Rv]] |
| - | [[Category: Goldstone, D C]]
| + | [[Category: Baker EN]] |
| - | [[Category: Metcalf, P]]
| + | [[Category: Goldstone DC]] |
| - | [[Category: XMTB, Mycobacterium Tuberculosis Structural Proteomics Project]]
| + | [[Category: Metcalf P]] |
| - | [[Category: Carbohydrate binding module]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Mycobacterium tuberculosis structural proteomics project]] | + | |
| - | [[Category: Psi-biology]] | + | |
| - | [[Category: Redox]] | + | |
| - | [[Category: Structural genomic]] | + | |
| - | [[Category: Thioredoxin]]
| + | |
| - | [[Category: Xmtb]]
| + | |
| Structural highlights
Function
DIPZ_MYCTU
Publication Abstract from PubMed
The members of the CcdA family are integral membrane proteins that use a disulfide cascade to transport electrons from the thioredoxin-thioredoxin reductase system in the interior of the cell into the extracytoplasmic space. The core transmembrane portion of this family is often elaborated with additional hydrophilic domains that act as adapters to deliver reducing potential to targets outside the cellular membrane. To investigate the function of family members in Mycobacterium tuberculosis, the structure of the C-terminal ectodomain from Rv2874, one of three CcdA-family members present in the genome, was determined. The crystal structure, which was refined at 1.9 A resolution with R = 0.195 and Rfree = 0.219, reveals the predicted thioredoxin-like domain with its conserved Cys-X-X-Cys active-site motif. Unexpectedly, this domain is combined with a second domain with a carbohydrate-binding module (CBM) fold, this being the first reported example of a CBM in association with a thioredoxin-like domain fold. A cavity in the CBM adjacent to the thioredoxin active site suggests a likely carbohydrate-binding site, representing a broadening of the substrate range for CcdA-family members and an expansion of the thioredoxin-domain functionality to carbohydrate modification.
Structure of the ectodomain of the electron transporter Rv2874 from Mycobacterium tuberculosis reveals a thioredoxin-like domain combined with a carbohydrate-binding module.,Goldstone DC, Metcalf P, Baker EN Acta Crystallogr D Struct Biol. 2016 Jan;72(Pt 1):40-8. doi:, 10.1107/S2059798315021488. Epub 2016 Jan 1. PMID:26894533[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Goldstone DC, Metcalf P, Baker EN. Structure of the ectodomain of the electron transporter Rv2874 from Mycobacterium tuberculosis reveals a thioredoxin-like domain combined with a carbohydrate-binding module. Acta Crystallogr D Struct Biol. 2016 Jan;72(Pt 1):40-8. doi:, 10.1107/S2059798315021488. Epub 2016 Jan 1. PMID:26894533 doi:http://dx.doi.org/10.1107/S2059798315021488
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