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| | ==Structure of the major structural protein D135 of Acidianus tailed spindle virus (ATSV)== | | ==Structure of the major structural protein D135 of Acidianus tailed spindle virus (ATSV)== |
| - | <StructureSection load='5eqw' size='340' side='right' caption='[[5eqw]], [[Resolution|resolution]] 1.68Å' scene=''> | + | <StructureSection load='5eqw' size='340' side='right'caption='[[5eqw]], [[Resolution|resolution]] 1.68Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5eqw]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Acidianus_tailed_spindle_virus Acidianus tailed spindle virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EQW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EQW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5eqw]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Acidianus_tailed_spindle_virus Acidianus tailed spindle virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EQW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EQW FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.679Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ATSV_D135 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1797140 Acidianus tailed spindle virus])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5eqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eqw OCA], [https://pdbe.org/5eqw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5eqw RCSB], [https://www.ebi.ac.uk/pdbsum/5eqw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5eqw ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5eqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eqw OCA], [http://pdbe.org/5eqw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5eqw RCSB], [http://www.ebi.ac.uk/pdbsum/5eqw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5eqw ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A125SJ78_9VIRU A0A125SJ78_9VIRU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Acidianus tailed spindle virus]] | | [[Category: Acidianus tailed spindle virus]] |
| - | [[Category: Hochstein, R A]] | + | [[Category: Large Structures]] |
| - | [[Category: Lawrence, C M]] | + | [[Category: Hochstein RA]] |
| - | [[Category: Lintner, N G]] | + | [[Category: Lawrence CM]] |
| - | [[Category: Young, M J]] | + | [[Category: Lintner NG]] |
| - | [[Category: Archaeal virus]] | + | [[Category: Young MJ]] |
| - | [[Category: Four helix bundle]]
| + | |
| - | [[Category: Large tailed spindle virus]]
| + | |
| - | [[Category: Structural protein]]
| + | |
| Structural highlights
Function
A0A125SJ78_9VIRU
Publication Abstract from PubMed
The spindle-shaped virion morphology is common among archaeal viruses, where it is a defining characteristic of many viral families. However, structural heterogeneity intrinsic to spindle-shaped viruses has seriously hindered efforts to elucidate the molecular architecture of these lemon-shaped capsids. We have utilized a combination of cryo-electron microscopy and X-ray crystallography to study Acidianus tailed spindle virus (ATSV). These studies reveal the architectural principles that underlie assembly of a spindle-shaped virus. Cryo-electron tomography shows a smooth transition from the spindle-shaped capsid into the tubular-shaped tail and allows low-resolution structural modeling of individual virions. Remarkably, higher-dose 2D micrographs reveal a helical surface lattice in the spindle-shaped capsid. Consistent with this, crystallographic studies of the major capsid protein reveal a decorated four-helix bundle that packs within the crystal to form a four-start helical assembly with structural similarity to the tube-shaped tail structure of ATSV and other tailed, spindle-shaped viruses. Combined, this suggests that the spindle-shaped morphology of the ATSV capsid is formed by a multistart helical assembly with a smoothly varying radius and allows construction of a pseudoatomic model for the lemon-shaped capsid that extends into a tubular tail. The potential advantages that this novel architecture conveys to the life cycle of spindle-shaped viruses, including a role in DNA ejection, are discussed.
Structural studies of Acidianus tailed spindle virus reveal a structural paradigm used in the assembly of spindle-shaped viruses.,Hochstein R, Bollschweiler D, Dharmavaram S, Lintner NG, Plitzko JM, Bruinsma R, Engelhardt H, Young MJ, Klug WS, Lawrence CM Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):2120-2125. doi:, 10.1073/pnas.1719180115. Epub 2018 Feb 12. PMID:29440399[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hochstein R, Bollschweiler D, Dharmavaram S, Lintner NG, Plitzko JM, Bruinsma R, Engelhardt H, Young MJ, Klug WS, Lawrence CM. Structural studies of Acidianus tailed spindle virus reveal a structural paradigm used in the assembly of spindle-shaped viruses. Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):2120-2125. doi:, 10.1073/pnas.1719180115. Epub 2018 Feb 12. PMID:29440399 doi:http://dx.doi.org/10.1073/pnas.1719180115
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