5ezm

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==Crystal Structure of ArnT from Cupriavidus metallidurans in the apo state==
==Crystal Structure of ArnT from Cupriavidus metallidurans in the apo state==
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<StructureSection load='5ezm' size='340' side='right' caption='[[5ezm]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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<StructureSection load='5ezm' size='340' side='right'caption='[[5ezm]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5ezm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cupmc Cupmc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EZM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EZM FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5ezm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cupriavidus_metallidurans_CH34 Cupriavidus metallidurans CH34]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EZM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EZM FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DSL:MONO-TRANS,+OCTA-CIS+DECAPRENYL-PHOSPHATE'>DSL</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=MPG:[(Z)-OCTADEC-9-ENYL]+(2R)-2,3-BIS(OXIDANYL)PROPANOATE'>MPG</scene>, <scene name='pdbligand=PC:PHOSPHOCHOLINE'>PC</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Rmet_4828 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=266264 CUPMC])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DSL:MONO-TRANS,+OCTA-CIS+DECAPRENYL-PHOSPHATE'>DSL</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=MPG:[(Z)-OCTADEC-9-ENYL]+(2R)-2,3-BIS(OXIDANYL)PROPANOATE'>MPG</scene>, <scene name='pdbligand=PC:PHOSPHOCHOLINE'>PC</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lipid_IV(A)_4-amino-4-deoxy-L-arabinosyltransferase Lipid IV(A) 4-amino-4-deoxy-L-arabinosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.43 2.4.2.43] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ezm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ezm OCA], [https://pdbe.org/5ezm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ezm RCSB], [https://www.ebi.ac.uk/pdbsum/5ezm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ezm ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ezm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ezm OCA], [http://pdbe.org/5ezm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ezm RCSB], [http://www.ebi.ac.uk/pdbsum/5ezm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ezm ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q1LDT6_CUPMC Q1LDT6_CUPMC]
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Polymyxins are antibiotics used in the last line of defense to combat multidrug-resistant infections by Gram-negative bacteria. Polymyxin resistance arises through charge modification of the bacterial outer membrane with the attachment of the cationic sugar 4-amino-4-deoxy-l-arabinose to lipid A, a reaction catalyzed by the integral membrane lipid-to-lipid glycosyltransferase 4-amino-4-deoxy-L-arabinose transferase (ArnT). Here, we report crystal structures of ArnT from Cupriavidus metallidurans, alone and in complex with the lipid carrier undecaprenyl phosphate, at 2.8 and 3.2 angstrom resolution, respectively. The structures show cavities for both lipidic substrates, which converge at the active site. A structural rearrangement occurs on undecaprenyl phosphate binding, which stabilizes the active site and likely allows lipid A binding. Functional mutagenesis experiments based on these structures suggest a mechanistic model for ArnT family enzymes.
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Structures of aminoarabinose transferase ArnT suggest a molecular basis for lipid A glycosylation.,Petrou VI, Herrera CM, Schultz KM, Clarke OB, Vendome J, Tomasek D, Banerjee S, Rajashankar KR, Belcher Dufrisne M, Kloss B, Kloppmann E, Rost B, Klug CS, Trent MS, Shapiro L, Mancia F Science. 2016 Feb 5;351(6273):608-12. doi: 10.1126/science.aad1172. PMID:26912703<ref>PMID:26912703</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5ezm" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Cupmc]]
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[[Category: Cupriavidus metallidurans CH34]]
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[[Category: Banerjee, S]]
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[[Category: Large Structures]]
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[[Category: Clarke, O B]]
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[[Category: Banerjee S]]
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[[Category: Mancia, F]]
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[[Category: Clarke OB]]
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[[Category: NYCOMPS, New York Consortium on Membrane Protein Structure]]
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[[Category: Mancia F]]
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[[Category: Petrou, V I]]
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[[Category: Petrou VI]]
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[[Category: Rajashankar, K R]]
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[[Category: Rajashankar KR]]
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[[Category: Tomasek, D]]
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[[Category: Tomasek D]]
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[[Category: Gt-c fold]]
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[[Category: Lipid glycosyltransferase]]
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[[Category: Membrane protein]]
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[[Category: New york consortium on membrane protein structure]]
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[[Category: Nycomp]]
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[[Category: Psi-biology]]
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[[Category: Structural genomic]]
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[[Category: Transferase]]
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[[Category: Zinc]]
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Current revision

Crystal Structure of ArnT from Cupriavidus metallidurans in the apo state

PDB ID 5ezm

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