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| - | [[Image:2h7o.gif|left|200px]] | |
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| - | {{Structure
| + | ==Crystal structure of the Rho-GTPase binding domain of YpkA== |
| - | |PDB= 2h7o |SIZE=350|CAPTION= <scene name='initialview01'>2h7o</scene>, resolution 2.000Å
| + | <StructureSection load='2h7o' size='340' side='right'caption='[[2h7o]], [[Resolution|resolution]] 2.00Å' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND=
| + | <table><tr><td colspan='2'>[[2h7o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_pseudotuberculosis Yersinia pseudotuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H7O FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | |GENE= ypkA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=633 Yersinia pseudotuberculosis])
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h7o OCA], [https://pdbe.org/2h7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h7o RCSB], [https://www.ebi.ac.uk/pdbsum/2h7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h7o ProSAT]</span></td></tr> |
| - | |DOMAIN=
| + | </table> |
| - | |RELATEDENTRY=
| + | == Function == |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h7o OCA], [http://www.ebi.ac.uk/pdbsum/2h7o PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2h7o RCSB]</span>
| + | [https://www.uniprot.org/uniprot/YPKA_YERPS YPKA_YERPS] Acts as a virulence determinant.<ref>PMID:10920208</ref> <ref>PMID:17531806</ref> |
| - | }}
| + | == References == |
| - | | + | <references/> |
| - | '''Crystal structure of the Rho-GTPase binding domain of YpkA'''
| + | __TOC__ |
| - | | + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | ==Overview==
| + | |
| - | Yersinia spp. cause gastroenteritis and the plague, representing historically devastating pathogens that are currently an important biodefense and antibiotic resistance concern. A critical virulence determinant is the Yersinia protein kinase A, or YpkA, a multidomain protein that disrupts the eukaryotic actin cytoskeleton. Here we solve the crystal structure of a YpkA-Rac1 complex and find that YpkA possesses a Rac1 binding domain that mimics host guanidine nucleotide dissociation inhibitors (GDIs) of the Rho GTPases. YpkA inhibits nucleotide exchange in Rac1 and RhoA, and mutations that disrupt the YpkA-GTPase interface abolish this activity in vitro and impair in vivo YpkA-induced cytoskeletal disruption. In cell culture experiments, the kinase and the GDI domains of YpkA act synergistically to promote cytoskeletal disruption, and a Y. pseudotuberculosis mutant lacking YpkA GDI activity shows attenuated virulence in a mouse infection assay. We conclude that virulence in Yersinia depends strongly upon mimicry of host GDI proteins by YpkA.
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| - | | + | |
| - | ==About this Structure==
| + | |
| - | 2H7O is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Yersinia_pseudotuberculosis Yersinia pseudotuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H7O OCA].
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| - | | + | |
| - | ==Reference== | + | |
| - | Yersinia virulence depends on mimicry of host Rho-family nucleotide dissociation inhibitors., Prehna G, Ivanov MI, Bliska JB, Stebbins CE, Cell. 2006 Sep 8;126(5):869-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16959567 16959567]
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| - | [[Category: Single protein]] | + | |
| | [[Category: Yersinia pseudotuberculosis]] | | [[Category: Yersinia pseudotuberculosis]] |
| - | [[Category: Bliska, J B.]] | + | [[Category: Bliska JB]] |
| - | [[Category: Ivanov, M.]] | + | [[Category: Ivanov M]] |
| - | [[Category: Prehna, G.]] | + | [[Category: Prehna G]] |
| - | [[Category: Stebbins, C E.]] | + | [[Category: Stebbins CE]] |
| - | [[Category: gdi]]
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| - | [[Category: yersinia]]
| + | |
| - | [[Category: yopo]]
| + | |
| - | [[Category: ypka]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:26:38 2008''
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