5uza

<StructureSection load='5uza' size='340' side='right' caption='[[5uza]], [[Resolution|resolution]] 2.22&Aring;' scene=''>

<table><tr><td colspan='2'>[[5uza]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UZA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UZA FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADE:ADENINE'>ADE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>

<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=IU:5-IODOURIDINE-5-MONOPHOSPHATE'>IU</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uza OCA], [http://pdbe.org/5uza PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uza RCSB], [http://www.ebi.ac.uk/pdbsum/5uza PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uza ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Site-specific incorporation of labeled nucleotides is an extremely useful synthetic tool for many structural studies (e.g., NMR, electron paramagnetic resonance (EPR), fluorescence resonance energy transfer (FRET), and X-ray crystallography) of RNA. However, specific-position-labeled RNAs &gt;60 nt are not commercially available on a milligram scale. Position-selective labeling of RNA (PLOR) has been applied to prepare large RNAs labeled at desired positions, and all the required reagents are commercially available. Here, we present a step-by-step protocol for the solid-liquid hybrid phase method PLOR to synthesize 71-nt RNA samples with three different modification applications, containing (i) a (13)C(15)N-labeled segment; (ii) discrete residues modified with Cy3, Cy5, or biotin; or (iii) two iodo-U residues. The flexible procedure enables a wide range of downstream biophysical analyses using precisely localized functionalized nucleotides. All three RNAs were obtained in &lt;2 d, excluding time for preparing reagents and optimizing experimental conditions. With optimization, the protocol can be applied to other RNAs with various labeling schemes, such as ligation of segmentally labeled fragments.

 

== References ==

[[Category: Liu, Y]]

[[Category: Stagno, J R]]

[[Category: Wang, Y X]]

[[Category: Adenine riboswitch]]

[[Category: Iodo-uridine]]

[[Category: Rna]]