1ymh

From Proteopedia

(Difference between revisions)

Current revision

anti-HCV Fab 19D9D6 complexed with protein L (PpL) mutant A66W

Structural highlights

1ymh is a 6 chain structure with sequence from Finegoldia magna ATCC 29328 and Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.6Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q51918_FINMA

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Protein L from Peptostreptococcus magnus (PpL) is a multidomain protein composed of four or five immunoglobulin-binding domains that target the kappa light chain of a large repertoire of human and murine antibodies. Thus, a single domain of this protein can be used to aid the crystallization of Fab, free or complexed to their antigen when it is not possible to obtain crystals without it. Each wild-type PpL domain has two light-chain binding sites that target the same region of the light chain and can thus bring together two Fab-antigen complexes within the crystal lattice. In this context the small PpL domain is sandwiched between two Fab and cannot participate in crystal contacts, thus mutants are unlikely to increase the chances of crystallizing a particular complex. However, it is possible to design mutants that can bind at only one site by making use of the crystal structures obtained so far. Such mutants will have a free surface that can participate in crystal contacts and that can be modified to improve its crystal contact-forming properties. Here, a comparison of two single-site mutants that differ at three different positions is reported. In both mutants two different tryptophan residues participate in crystal-packing interactions, suggesting that this residue may be particularly interesting for enhancing crystal-contact formation.

Comparison of the crystallization and crystal packing of two Fab single-site mutant protein L complexes.,Granata V, Housden NG, Harrison S, Jolivet-Reynaud C, Gore MG, Stura EA Acta Crystallogr D Biol Crystallogr. 2005 Jun;61(Pt 6):750-4. Epub 2005, May 26. PMID:15930633^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Granata V, Housden NG, Harrison S, Jolivet-Reynaud C, Gore MG, Stura EA. Comparison of the crystallization and crystal packing of two Fab single-site mutant protein L complexes. Acta Crystallogr D Biol Crystallogr. 2005 Jun;61(Pt 6):750-4. Epub 2005, May 26. PMID:15930633 doi:http://dx.doi.org/10.1107/S0907444905007110

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ymh"

@@ Line 1: / Line 1: @@
 ==anti-HCV Fab 19D9D6 complexed with protein L (PpL) mutant A66W==
-<StructureSection load='1ymh' size='340' side='right' caption='[[1ymh]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
+<StructureSection load='1ymh' size='340' side='right'caption='[[1ymh]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1ymh]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Finm2 Finm2] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YMH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YMH FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1ymh]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Finegoldia_magna_ATCC_29328 Finegoldia magna ATCC 29328] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YMH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YMH FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ymh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ymh OCA], [http://pdbe.org/1ymh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ymh RCSB], [http://www.ebi.ac.uk/pdbsum/1ymh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ymh ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ymh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ymh OCA], [https://pdbe.org/1ymh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ymh RCSB], [https://www.ebi.ac.uk/pdbsum/1ymh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ymh ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q51918_FINMA Q51918_FINMA]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 11: / Line 14: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ym/1ymh_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 32: / Line 35: @@
 __TOC__
 </StructureSection>
-[[Category: Finm2]]
+[[Category: Finegoldia magna ATCC 29328]]
+[[Category: Large Structures]]
 [[Category: Mus musculus]]
-[[Category: Gore, M G]]
+[[Category: Gore MG]]
-[[Category: Granata, V]]
+[[Category: Granata V]]
-[[Category: Harrison, S]]
+[[Category: Harrison S]]
-[[Category: Housden, N G]]
+[[Category: Housden NG]]
-[[Category: Jolivet-Reynaud, C]]
+[[Category: Jolivet-Reynaud C]]
-[[Category: Stura, E A]]
+[[Category: Stura EA]]
-[[Category: Engineering of crystal contact]]
-[[Category: Immune system]]
-[[Category: Ppl-fab complex]]

1ymh

From Proteopedia

Current revision

anti-HCV Fab 19D9D6 complexed with protein L (PpL) mutant A66W

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox