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| | ==NMR structure of Mistic== | | ==NMR structure of Mistic== |
| - | <StructureSection load='1ygm' size='340' side='right' caption='[[1ygm]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='1ygm' size='340' side='right'caption='[[1ygm]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1ygm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YGM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1ygm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YGM FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">YugO-a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ygm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ygm OCA], [http://pdbe.org/1ygm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ygm RCSB], [http://www.ebi.ac.uk/pdbsum/1ygm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ygm ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ygm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ygm OCA], [https://pdbe.org/1ygm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ygm RCSB], [https://www.ebi.ac.uk/pdbsum/1ygm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ygm ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MSTX_BACSU MSTX_BACSU]] Chaperone that facilitates the production and integration of integral membrane proteins into the bacterial lipid bilayer.<ref>PMID:15731457</ref> | + | [https://www.uniprot.org/uniprot/MSTX_BACSU MSTX_BACSU] Chaperone that facilitates the production and integration of integral membrane proteins into the bacterial lipid bilayer.<ref>PMID:15731457</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacsu]] | + | [[Category: Bacillus subtilis subsp. subtilis str. 168]] |
| - | [[Category: Castronovo, S]] | + | [[Category: Large Structures]] |
| - | [[Category: Choe, S]] | + | [[Category: Castronovo S]] |
| - | [[Category: Greenwald, J]] | + | [[Category: Choe S]] |
| - | [[Category: Riek, R]] | + | [[Category: Greenwald J]] |
| - | [[Category: Roosild, T P]] | + | [[Category: Riek R]] |
| - | [[Category: Vega, M]] | + | [[Category: Roosild TP]] |
| - | [[Category: Alpha-helical bundle]]
| + | [[Category: Vega M]] |
| - | [[Category: Integral membrane protein]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| Structural highlights
Function
MSTX_BACSU Chaperone that facilitates the production and integration of integral membrane proteins into the bacterial lipid bilayer.[1]
Publication Abstract from PubMed
Although structure determination of soluble proteins has become routine, our understanding of membrane proteins has been limited by experimental bottlenecks in obtaining both sufficient yields of protein and ordered crystals. Mistic is an unusual Bacillus subtilis integral membrane protein that folds autonomously into the membrane, bypassing the cellular translocon machinery. Using paramagnetic probes, we determined by nuclear magnetic resonance (NMR) spectroscopy that the protein forms a helical bundle with a surprisingly polar lipid-facing surface. Additional experiments suggest that Mistic can be used for high-level production of other membrane proteins in their native conformations, including many eukaryotic proteins that have previously been intractable to bacterial expression.
NMR structure of Mistic, a membrane-integrating protein for membrane protein expression.,Roosild TP, Greenwald J, Vega M, Castronovo S, Riek R, Choe S Science. 2005 Feb 25;307(5713):1317-21. PMID:15731457[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Roosild TP, Greenwald J, Vega M, Castronovo S, Riek R, Choe S. NMR structure of Mistic, a membrane-integrating protein for membrane protein expression. Science. 2005 Feb 25;307(5713):1317-21. PMID:15731457 doi:http://dx.doi.org/307/5713/1317
- ↑ Roosild TP, Greenwald J, Vega M, Castronovo S, Riek R, Choe S. NMR structure of Mistic, a membrane-integrating protein for membrane protein expression. Science. 2005 Feb 25;307(5713):1317-21. PMID:15731457 doi:http://dx.doi.org/307/5713/1317
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