5vpp

Publication Abstract from PubMed

Accurate translation of the genetic code is critical to ensure expression of proteins with correct amino acid sequences. Certain tRNAs can cause a shift out of frame (i.e., frameshifting) due to imbalances in tRNA concentrations, lack of tRNA modifications or insertions or deletions in tRNAs (called frameshift suppressors). Here, we determined the structural basis for how frameshift-suppressor tRNA(SufA6) (a derivative of tRNA(Pro)) reprograms the mRNA frame to translate a 4-nt codon when bound to the bacterial ribosome. After decoding at the aminoacyl (A) site, the crystal structure of the anticodon stem-loop of tRNA(SufA6) bound in the peptidyl (P) site reveals ASL conformational changes that allow for recoding into the +1 mRNA frame. Furthermore, a crystal structure of full-length tRNA(SufA6) programmed in the P site shows extensive conformational rearrangements of the 30S head and body domains similar to what is observed in a translocation intermediate state containing elongation factor G (EF-G). The 30S movement positions tRNA(SufA6) toward the 30S exit (E) site disrupting key 16S rRNA-mRNA interactions that typically define the mRNA frame. In summary, this tRNA-induced 30S domain change in the absence of EF-G causes the ribosome to lose its grip on the mRNA and uncouples the canonical forward movement of the tRNAs during elongation.

Mechanism of tRNA-mediated +1 ribosomal frameshifting.,Hong S, Sunita S, Maehigashi T, Hoffer ED, Dunkle JA, Dunham CM Proc Natl Acad Sci U S A. 2018 Sep 27. pii: 1809319115. doi:, 10.1073/pnas.1809319115. PMID:30262649^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.