6gdg
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6gdg is ON HOLD Authors: Garcia-Nafria, J., Lee, Y. Description: Cryo-EM structure of the adenosine A2A receptor bound to a miniGs heterotrimer [[C...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Cryo-EM structure of the adenosine A2A receptor bound to a miniGs heterotrimer== | |
| + | <SX load='6gdg' size='340' side='right' viewer='molstar' caption='[[6gdg]], [[Resolution|resolution]] 4.11Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6gdg]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GDG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GDG FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.11Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NEC:N-ETHYL-5-CARBOXAMIDO+ADENOSINE'>NEC</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gdg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gdg OCA], [https://pdbe.org/6gdg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gdg RCSB], [https://www.ebi.ac.uk/pdbsum/6gdg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gdg ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/THIO_ECOLI THIO_ECOLI] Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions.[https://www.uniprot.org/uniprot/AA2AR_HUMAN AA2AR_HUMAN] Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The adenosine A2A receptor (A2AR) is a prototypical G protein-coupled receptor (GPCR) that couples to the heterotrimeric G protein GS. Here we determine the structure by electron cryo-microscopy (cryo-EM) of A2AR at pH 7.5 bound to the small molecule agonist NECA and coupled to an engineered heterotrimeric G protein, which contains mini-GS, the betagamma subunits and nanobody Nb35. Most regions of the complex have a resolution of ~3.8 A or better. Comparison with the 3.4 A resolution crystal structure shows that the receptor and mini-GS are virtually identical and that the density of the side chains and ligand are of comparable quality. However, the cryo-EM density map also indicates regions that are flexible in comparison to the crystal structures, which unexpectedly includes regions in the ligand binding pocket. In addition, an interaction between intracellular loop 1 of the receptor and the beta subunit of the G protein was observed. | ||
| - | + | Cryo-EM structure of the adenosine A2A receptor coupled to an engineered heterotrimeric G protein.,Garcia-Nafria J, Lee Y, Bai X, Carpenter B, Tate CG Elife. 2018 May 4;7. pii: 35946. doi: 10.7554/eLife.35946. PMID:29726815<ref>PMID:29726815</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Garcia-Nafria | + | <div class="pdbe-citations 6gdg" style="background-color:#fffaf0;"></div> |
| - | [[Category: Lee | + | |
| + | ==See Also== | ||
| + | *[[Adenosine A2A receptor 3D structures|Adenosine A2A receptor 3D structures]] | ||
| + | *[[Transducin 3D structures|Transducin 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </SX> | ||
| + | [[Category: Escherichia coli]] | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Lama glama]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Garcia-Nafria J]] | ||
| + | [[Category: Lee Y]] | ||
Current revision
Cryo-EM structure of the adenosine A2A receptor bound to a miniGs heterotrimer
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