|
|
| (One intermediate revision not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Structure of PsDef1 defensin from Pinus sylvestris== | | ==Structure of PsDef1 defensin from Pinus sylvestris== |
| - | <StructureSection load='5nce' size='340' side='right' caption='[[5nce]], [[NMR_Ensembles_of_Models | 15 NMR models]]' scene=''> | + | <StructureSection load='5nce' size='340' side='right'caption='[[5nce]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5nce]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pinsy Pinsy]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NCE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NCE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5nce]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pinus_sylvestris Pinus sylvestris]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NCE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NCE FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Def1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3349 PINSY])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 15 models</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nce FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nce OCA], [http://pdbe.org/5nce PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nce RCSB], [http://www.ebi.ac.uk/pdbsum/5nce PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nce ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nce FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nce OCA], [https://pdbe.org/5nce PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nce RCSB], [https://www.ebi.ac.uk/pdbsum/5nce PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nce ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/DEF1_PINSY DEF1_PINSY] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 16: |
Line 18: |
| | </div> | | </div> |
| | <div class="pdbe-citations 5nce" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5nce" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Defensin 3D structures|Defensin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Pinsy]] | + | [[Category: Large Structures]] |
| - | [[Category: Bessolitsyna, E K]] | + | [[Category: Pinus sylvestris]] |
| - | [[Category: Ermakova, E A]] | + | [[Category: Bessolitsyna EK]] |
| - | [[Category: Khairutdinov, B I]] | + | [[Category: Ermakova EA]] |
| - | [[Category: Kovaleva, V]] | + | [[Category: Khairutdinov BI]] |
| - | [[Category: Nesmelova, I V]] | + | [[Category: Kovaleva V]] |
| - | [[Category: Tarasova, N B]] | + | [[Category: Nesmelova IV]] |
| - | [[Category: Toporkova, Y Y]] | + | [[Category: Tarasova NB]] |
| - | [[Category: Zuev, Y F]] | + | [[Category: Toporkova YY]] |
| - | [[Category: Amylase inhibition]]
| + | [[Category: Zuev YF]] |
| - | [[Category: Antibacterial activity]]
| + | |
| - | [[Category: Conformational dynamic]]
| + | |
| - | [[Category: Defensin]]
| + | |
| - | [[Category: Plant defensin]]
| + | |
| - | [[Category: Plant protein]]
| + | |
| - | [[Category: Scotts pine defensin]]
| + | |
| Structural highlights
Function
DEF1_PINSY
Publication Abstract from PubMed
Plants have developed a complex defense response system against pests and pathogens. Defensins, produced by plants as part of their innate immune response, form the family of small, basic, cysteine-rich proteins with activity primarily directed against fungal pathogens. In addition, plant defensins can show antibacterial activity and protease and insect amylase inhibitory activities. However, in gymnosperms, only antifungal activity of defensins has been described thus far. Here, we report antibacterial and insect alpha-amylase inhibition activities for defensin PsDef1 from P. sylvestris, the first defensin from gymnosperms with a broad range of biological activities described. We also report the solution NMR structure of PsDef1 and its dynamics properties assessed by a combination of experimental NMR and computational techniques. Collectively, our data provide an insight into structure, dynamics, and functional properties of PsDef1 that could be common between defensins from this taxonomic group.
NMR structure, conformational dynamics, and biological activity of PsDef1 defensin from Pinus sylvestris.,Khairutdinov BI, Ermakova EA, Yusypovych YM, Bessolicina EK, Tarasova NB, Toporkova YY, Kovaleva V, Zuev YF, Nesmelova IV Biochim Biophys Acta. 2017 Aug;1865(8):1085-1094. doi:, 10.1016/j.bbapap.2017.05.012. Epub 2017 May 17. PMID:28528214[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Khairutdinov BI, Ermakova EA, Yusypovych YM, Bessolicina EK, Tarasova NB, Toporkova YY, Kovaleva V, Zuev YF, Nesmelova IV. NMR structure, conformational dynamics, and biological activity of PsDef1 defensin from Pinus sylvestris. Biochim Biophys Acta. 2017 Aug;1865(8):1085-1094. doi:, 10.1016/j.bbapap.2017.05.012. Epub 2017 May 17. PMID:28528214 doi:http://dx.doi.org/10.1016/j.bbapap.2017.05.012
|
