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| | ==Crystal structure of QscR bound to C12-homoserine lactone== | | ==Crystal structure of QscR bound to C12-homoserine lactone== |
| - | <StructureSection load='6cc0' size='340' side='right' caption='[[6cc0]], [[Resolution|resolution]] 2.50Å' scene=''> | + | <StructureSection load='6cc0' size='340' side='right'caption='[[6cc0]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6cc0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CC0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CC0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6cc0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CC0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CC0 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EWM:N-[(3S)-2-oxooxolan-3-yl]dodecanamide'>EWM</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3szt|3szt]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EWM:N-[(3S)-2-oxooxolan-3-yl]dodecanamide'>EWM</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">phzR, qscR, sdiA_2, CAZ03_14830, CAZ10_26210, DC19_16645, HQ52_16845, PAERUG_E15_London_28_01_14_06284, PAMH19_5306 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cc0 OCA], [https://pdbe.org/6cc0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cc0 RCSB], [https://www.ebi.ac.uk/pdbsum/6cc0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cc0 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cc0 OCA], [http://pdbe.org/6cc0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cc0 RCSB], [http://www.ebi.ac.uk/pdbsum/6cc0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cc0 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q9RMS5_PSEAI Q9RMS5_PSEAI] |
| - | Pseudomonas aeruginosa is an opportunistic pathogen that uses the process of quorum sensing (QS) to coordinate the expression of many virulence genes. During quorum sensing, N-acyl-homoserine lactone (AHL) signaling molecules regulate the activity of three LuxR-type transcription factors, LasR, RhlR, and QscR. To better understand P. aeruginosa QS signal reception, we examined the mechanism underlying the response of QscR to synthetic agonists and antagonists using biophysical and structural approaches. The structure of QscR bound to a synthetic agonist reveals a novel mode of ligand binding supporting a general mechanism for agonist activity. In turn, antagonists of QscR with partial agonist activity were found to destabilize and greatly impair QscR dimerization and DNA binding. These results highlight the diversity of LuxR-type receptor responses to small molecule agonists and antagonists and demonstrate the potential for chemical strategies for the selective targeting of individual quorum-sensing systems. This article is protected by copyright. All rights reserved.
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| - | Mechanism of agonism and antagonism of the Pseudomonas aeruginosa quorum sensing regulator QscR with non-native ligands.,Wysoczynski-Horita CL, Boursier ME, Hill R, Hansen K, Blackwell HE, Churchill MEA Mol Microbiol. 2018 Feb 13. doi: 10.1111/mmi.13930. PMID:29437248<ref>PMID:29437248</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 6cc0" style="background-color:#fffaf0;"></div>
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| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Churchill, M E.A]] | + | [[Category: Large Structures]] |
| - | [[Category: Wysoczynski-Horita, C L]] | + | [[Category: Pseudomonas aeruginosa]] |
| - | [[Category: Luxr-type ahl receptor]] | + | [[Category: Churchill MEA]] |
| - | [[Category: Pseudomonas aeruginosa qscr]] | + | [[Category: Wysoczynski-Horita CL]] |
| - | [[Category: Transcription]]
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