1zn2
From Proteopedia
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==Low Resolution Structure of Response Regulator StyR== | ==Low Resolution Structure of Response Regulator StyR== | ||
| - | <StructureSection load='1zn2' size='340' side='right' caption='[[1zn2]], [[Resolution|resolution]] 2.91Å' scene=''> | + | <StructureSection load='1zn2' size='340' side='right'caption='[[1zn2]], [[Resolution|resolution]] 2.91Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[1zn2]] is a 1 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[1zn2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZN2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZN2 FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.91Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zn2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zn2 OCA], [https://pdbe.org/1zn2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zn2 RCSB], [https://www.ebi.ac.uk/pdbsum/1zn2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zn2 ProSAT]</span></td></tr> |
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/O30989_PSEFL O30989_PSEFL] | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zn2 ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zn2 ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | StyR belongs to the FixJ subfamily of signal transduction response regulators; it controls transcription of the styABCD operon coding for styrene catabolism in Pseudomonas fluorescens ST. The crystal structure of unphosphorylated StyR is reported at 2.2 A resolution. StyR is composed of an N-terminal regulatory domain (StyR-N) and a C-terminal DNA binding domain (StyR-C). The two domains are separated by an elongated linker alpha helix (34 residues), a new feature in known response regulator structures. StyR-C is structured similarly to the DNA binding domain of the response regulator NarL. StyR-N shows structural reorganization of the phosphate receiving region involved in activation/homodimerization: specific residues adopt an "active-like" conformation, and the alpha4 helix, involved in dimerization of the homologous FixJ response regulator, is trimmed to just one helical turn. Overall, structural considerations suggest that phosphorylation may act as an allosteric switch, shifting a preexisting StyR equilibrium toward the active, dimeric, DNA binding form. | ||
| - | + | ==See Also== | |
| - | + | *[[Response regulator 3D structure|Response regulator 3D structure]] | |
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: Ascenzi | + | [[Category: Pseudomonas fluorescens]] |
| - | [[Category: Bolognesi | + | [[Category: Ascenzi P]] |
| - | [[Category: Leoni | + | [[Category: Bolognesi M]] |
| - | [[Category: Milani | + | [[Category: Leoni L]] |
| - | [[Category: Rampioni | + | [[Category: Milani M]] |
| - | [[Category: Zennaro | + | [[Category: Rampioni G]] |
| - | + | [[Category: Zennaro E]] | |
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Current revision
Low Resolution Structure of Response Regulator StyR
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