6gen
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6gen is ON HOLD Authors: Willhoft, O., Chua, E.Y.D., Wilkinson, M., Wigley, D.B. Description: Chromatin remodeller-nucleosome complex at 4.5 A reso...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Chromatin remodeller-nucleosome complex at 4.5 A resolution.== | |
| + | <SX load='6gen' size='340' side='right' viewer='molstar' caption='[[6gen]], [[Resolution|resolution]] 3.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6gen]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GEN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GEN FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.6Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gen FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gen OCA], [https://pdbe.org/6gen PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gen RCSB], [https://www.ebi.ac.uk/pdbsum/6gen PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gen ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The yeast SWR1 complex exchanges histone H2A in nucleosomes with Htz1 (H2A.Z in humans). The cryo-electron microscopy structure of the SWR1 complex bound to a nucleosome at 3.6-angstrom resolution reveals details of the intricate interactions between components of the SWR1 complex and its nucleosome substrate. Interactions between the Swr1 motor domains and the DNA wrap at superhelical location 2 distort the DNA, causing a bulge with concomitant translocation of the DNA by one base pair, coupled to conformational changes of the histone core. Furthermore, partial unwrapping of the DNA from the histone core takes place upon binding of nucleosomes to SWR1 complex. The unwrapping, as monitored by single-molecule data, is stabilized and has its dynamics altered by adenosine triphosphate binding but does not require hydrolysis. | ||
| - | + | Structure and dynamics of the yeast SWR1-nucleosome complex.,Willhoft O, Ghoneim M, Lin CL, Chua EYD, Wilkinson M, Chaban Y, Ayala R, McCormack EA, Ocloo L, Rueda DS, Wigley DB Science. 2018 Oct 12;362(6411). pii: 362/6411/eaat7716. doi:, 10.1126/science.aat7716. PMID:30309918<ref>PMID:30309918</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6gen" style="background-color:#fffaf0;"></div> |
| - | [[Category: Chua | + | |
| - | [[Category: Wigley | + | ==See Also== |
| - | [[Category: Willhoft | + | *[[Actin-related protein 3D structures|Actin-related protein 3D structures]] |
| + | *[[Helicase 3D structures|Helicase 3D structures]] | ||
| + | *[[Histone 3D structures|Histone 3D structures]] | ||
| + | *[[Vacuolar protein sorting-associated protein 3D structures|Vacuolar protein sorting-associated protein 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </SX> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Saccharomyces cerevisiae S288C]] | ||
| + | [[Category: Synthetic construct]] | ||
| + | [[Category: Chua EYD]] | ||
| + | [[Category: Wigley DB]] | ||
| + | [[Category: Wilkinson M]] | ||
| + | [[Category: Willhoft O]] | ||
Current revision
Chromatin remodeller-nucleosome complex at 4.5 A resolution.
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