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| | ==Crystal Structure of rice D14 bound to 2-(2-methyl-3-nitroanilino)benzoic acid== | | ==Crystal Structure of rice D14 bound to 2-(2-methyl-3-nitroanilino)benzoic acid== |
| - | <StructureSection load='6ap8' size='340' side='right' caption='[[6ap8]], [[Resolution|resolution]] 1.27Å' scene=''> | + | <StructureSection load='6ap8' size='340' side='right'caption='[[6ap8]], [[Resolution|resolution]] 1.27Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6ap8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AP8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AP8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6ap8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6AP8 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BNY:2-[(2-methyl-3-nitrophenyl)amino]benzoic+acid'>BNY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.27Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3w04|3w04]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BNY:2-[(2-methyl-3-nitrophenyl)amino]benzoic+acid'>BNY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ap8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ap8 OCA], [http://pdbe.org/6ap8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ap8 RCSB], [http://www.ebi.ac.uk/pdbsum/6ap8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ap8 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ap8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ap8 OCA], [https://pdbe.org/6ap8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ap8 RCSB], [https://www.ebi.ac.uk/pdbsum/6ap8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ap8 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/D14_ORYSJ D14_ORYSJ]] Involved in strigolactone signaling pathway. May function downstream of strigolactone synthesis, as a component of hormone signaling or as an enzyme that participates in the conversion of strigolactones to the bioactive form. Strigolactones are hormones that inhibit tillering and shoot branching through the MAX-dependent pathway, contribute to the regulation of shoot architectural response to phosphate-limiting conditions and function as rhizosphere signal that stimulates hyphal branching of arbuscular mycorrhizal fungi and trigger seed germination of root parasitic weeds.<ref>PMID:19542179</ref> <ref>PMID:23301669</ref> | + | [https://www.uniprot.org/uniprot/D14_ORYSJ D14_ORYSJ] Involved in strigolactone signaling pathway. May function downstream of strigolactone synthesis, as a component of hormone signaling or as an enzyme that participates in the conversion of strigolactones to the bioactive form. Strigolactones are hormones that inhibit tillering and shoot branching through the MAX-dependent pathway, contribute to the regulation of shoot architectural response to phosphate-limiting conditions and function as rhizosphere signal that stimulates hyphal branching of arbuscular mycorrhizal fungi and trigger seed germination of root parasitic weeds.<ref>PMID:19542179</ref> <ref>PMID:23301669</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Hamiaux, C]] | + | [[Category: Large Structures]] |
| - | [[Category: Alpha/beta hydrolase]] | + | [[Category: Oryza sativa Japonica Group]] |
| - | [[Category: Plant protein]] | + | [[Category: Hamiaux C]] |
| Structural highlights
Function
D14_ORYSJ Involved in strigolactone signaling pathway. May function downstream of strigolactone synthesis, as a component of hormone signaling or as an enzyme that participates in the conversion of strigolactones to the bioactive form. Strigolactones are hormones that inhibit tillering and shoot branching through the MAX-dependent pathway, contribute to the regulation of shoot architectural response to phosphate-limiting conditions and function as rhizosphere signal that stimulates hyphal branching of arbuscular mycorrhizal fungi and trigger seed germination of root parasitic weeds.[1] [2]
Publication Abstract from PubMed
The strigolactone (SL) family of plant hormones regulates a broad range of physiological processes affecting plant growth and development and also plays essential roles in controlling interactions with parasitic weeds and symbiotic fungi. Recent progress elucidating details of SL biosynthesis, signalling, and transport offer many opportunities for discovering new plant growth regulators via chemical interference. Here, using high throughput screening and downstream biochemical assays, we identified N-phenylanthranilic acid derivatives as potent inhibitors of the SL receptors from petunia (DAD2), rice (OsD14) and Arabidopsis (AtD14). Crystal structures of DAD2 and OsD14 in complex with inhibitors further provided detailed insights into the inhibition mechanism, and in silico modeling of 19 other plant strigolactone receptors suggested that these compounds are active across a large range of plant species. Altogether, these results provide chemical tools for investigating SL signaling and further define a framework for structure-based approaches to design and validate optimized inhibitors of SL receptors for specific plant targets.
Inhibition of strigolactone receptors by N-phenylanthranilic acid derivatives: structural and functional insights.,Hamiaux C, Drummond RSM, Luo Z, Lee HW, Sharma P, Janssen BJ, Perry NB, Denny WA, Snowden KC J Biol Chem. 2018 Mar 9. pii: RA117.001154. doi: 10.1074/jbc.RA117.001154. PMID:29523686[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Arite T, Umehara M, Ishikawa S, Hanada A, Maekawa M, Yamaguchi S, Kyozuka J. d14, a strigolactone-insensitive mutant of rice, shows an accelerated outgrowth of tillers. Plant Cell Physiol. 2009 Aug;50(8):1416-24. doi: 10.1093/pcp/pcp091. Epub 2009, Jun 19. PMID:19542179 doi:http://dx.doi.org/10.1093/pcp/pcp091
- ↑ Kagiyama M, Hirano Y, Mori T, Kim SY, Kyozuka J, Seto Y, Yamaguchi S, Hakoshima T. Structures of D14 and D14L in the strigolactone and karrikin signaling pathways. Genes Cells. 2013 Jan 10. doi: 10.1111/gtc.12025. PMID:23301669 doi:10.1111/gtc.12025
- ↑ Hamiaux C, Drummond RSM, Luo Z, Lee HW, Sharma P, Janssen BJ, Perry NB, Denny WA, Snowden KC. Inhibition of strigolactone receptors by N-phenylanthranilic acid derivatives: structural and functional insights. J Biol Chem. 2018 Mar 9. pii: RA117.001154. doi: 10.1074/jbc.RA117.001154. PMID:29523686 doi:http://dx.doi.org/10.1074/jbc.RA117.001154
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