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| | ==Solution NMR Structure of the Periplasmic Signaling Domain of the Outer Membrane Iron Transporter PupA from Pseudomonas putida.== | | ==Solution NMR Structure of the Periplasmic Signaling Domain of the Outer Membrane Iron Transporter PupA from Pseudomonas putida.== |
| - | <StructureSection load='2a02' size='340' side='right' caption='[[2a02]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2a02' size='340' side='right'caption='[[2a02]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2a02]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A02 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A02 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2a02]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A02 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A02 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1zzv|1zzv]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pupA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 "Bacillus fluorescens putidus" Flugge 1886])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a02 OCA], [https://pdbe.org/2a02 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a02 RCSB], [https://www.ebi.ac.uk/pdbsum/2a02 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a02 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a02 OCA], [http://pdbe.org/2a02 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2a02 RCSB], [http://www.ebi.ac.uk/pdbsum/2a02 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2a02 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PUPA_PSEPU PUPA_PSEPU]] Specific receptor for the siderophore ferric pseudobactin 358. | + | [https://www.uniprot.org/uniprot/PUPA_PSEPU PUPA_PSEPU] Specific receptor for the siderophore ferric pseudobactin 358. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus fluorescens putidus flugge 1886]] | + | [[Category: Large Structures]] |
| - | [[Category: Amezcua, C A]] | + | [[Category: Pseudomonas putida]] |
| - | [[Category: Chelliah, Y]] | + | [[Category: Amezcua CA]] |
| - | [[Category: Deisenhofer, J]] | + | [[Category: Chelliah Y]] |
| - | [[Category: Ferguson, A D]] | + | [[Category: Deisenhofer J]] |
| - | [[Category: Rosen, M K]] | + | [[Category: Ferguson AD]] |
| - | [[Category: Membrane protein]]
| + | [[Category: Rosen MK]] |
| - | [[Category: Metal transport]]
| + | |
| - | [[Category: Protein nmr]]
| + | |
| Structural highlights
Function
PUPA_PSEPU Specific receptor for the siderophore ferric pseudobactin 358.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Transcription of the ferric citrate import system is regulated by ferric citrate binding to the outer membrane transporter FecA. A signal indicating transporter occupancy is relayed across the outer membrane to energy-transducing and regulatory proteins embedded in the cytoplasmic membrane. Because transcriptional activation is not coupled to ferric citrate import, an allosteric mechanism underlies this complex signaling mechanism. Using evolution-based statistical analysis we have identified a sparse but structurally connected network of residues that links distant functional sites in FecA. Functional analyses of these positions confirm their involvement in the mechanism that regulates transcriptional activation in response to ferric citrate binding at the cell surface. This mechanism appears to be conserved and provides the structural basis for the allosteric signaling of TonB-dependent transporters.
Signal transduction pathway of TonB-dependent transporters.,Ferguson AD, Amezcua CA, Halabi NM, Chelliah Y, Rosen MK, Ranganathan R, Deisenhofer J Proc Natl Acad Sci U S A. 2007 Jan 9;104(2):513-8. Epub 2006 Dec 29. PMID:17197416[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ferguson AD, Amezcua CA, Halabi NM, Chelliah Y, Rosen MK, Ranganathan R, Deisenhofer J. Signal transduction pathway of TonB-dependent transporters. Proc Natl Acad Sci U S A. 2007 Jan 9;104(2):513-8. Epub 2006 Dec 29. PMID:17197416
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