2i06

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[[Image:2i06.gif|left|200px]]
 
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{{Structure
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==Escherichia Coli Replication Terminator Protein (Tus) Complexed With DNA- Locked form==
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|PDB= 2i06 |SIZE=350|CAPTION= <scene name='initialview01'>2i06</scene>, resolution 2.20&Aring;
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<StructureSection load='2i06' size='340' side='right'caption='[[2i06]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>
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<table><tr><td colspan='2'>[[2i06]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I06 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I06 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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|GENE= tus ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i06 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i06 OCA], [https://pdbe.org/2i06 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i06 RCSB], [https://www.ebi.ac.uk/pdbsum/2i06 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i06 ProSAT]</span></td></tr>
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|RELATEDENTRY=[[2i05|2I05]], [[2ewj|2EWJ]], [[1ecr|1ECR]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i06 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i06 OCA], [http://www.ebi.ac.uk/pdbsum/2i06 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2i06 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/TUS_ECOLI TUS_ECOLI] Trans-acting protein required for termination of DNA replication. Binds to DNA replication terminator sequences (terA to terF) to prevent the passage of replication forks. The termination efficiency will be affected by the affinity of this protein for the terminator sequence.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i0/2i06_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i06 ConSurf].
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<div style="clear:both"></div>
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'''Escherichia Coli Replication Terminator Protein (Tus) Complexed With DNA- Locked form'''
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==See Also==
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*[[Replication Termination Protein|Replication Termination Protein]]
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__TOC__
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==Overview==
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</StructureSection>
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During chromosome synthesis in Escherichia coli, replication forks are blocked by Tus bound Ter sites on approach from one direction but not the other. To study the basis of this polarity, we measured the rates of dissociation of Tus from forked TerB oligonucleotides, such as would be produced by the replicative DnaB helicase at both the fork-blocking (nonpermissive) and permissive ends of the Ter site. Strand separation of a few nucleotides at the permissive end was sufficient to force rapid dissociation of Tus to allow fork progression. In contrast, strand separation extending to and including the strictly conserved G-C(6) base pair at the nonpermissive end led to formation of a stable locked complex. Lock formation specifically requires the cytosine residue, C(6). The crystal structure of the locked complex showed that C(6) moves 14 A from its normal position to bind in a cytosine-specific pocket on the surface of Tus.
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==About this Structure==
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2I06 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I06 OCA].
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==Reference==
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A molecular mousetrap determines polarity of termination of DNA replication in E. coli., Mulcair MD, Schaeffer PM, Oakley AJ, Cross HF, Neylon C, Hill TM, Dixon NE, Cell. 2006 Jun 30;125(7):1309-19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16814717 16814717]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Dixon, N E.]]
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[[Category: Dixon NE]]
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[[Category: Mulcair, M D.]]
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[[Category: Mulcair MD]]
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[[Category: Oakley, A J.]]
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[[Category: Oakley AJ]]
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[[Category: Schaeffer, P M.]]
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[[Category: Schaeffer PM]]
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[[Category: protein-dna complex]]
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[[Category: replication/dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:37:51 2008''
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Current revision

Escherichia Coli Replication Terminator Protein (Tus) Complexed With DNA- Locked form

PDB ID 2i06

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