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| | ==SPRY domain-containing SOCS box protein 2 (SSB-2)== | | ==SPRY domain-containing SOCS box protein 2 (SSB-2)== |
| - | <StructureSection load='2afj' size='340' side='right' caption='[[2afj]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2afj' size='340' side='right'caption='[[2afj]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2afj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AFJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2AFJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2afj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AFJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AFJ FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Ssb-2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2afj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2afj OCA], [http://pdbe.org/2afj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2afj RCSB], [http://www.ebi.ac.uk/pdbsum/2afj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2afj ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2afj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2afj OCA], [https://pdbe.org/2afj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2afj RCSB], [https://www.ebi.ac.uk/pdbsum/2afj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2afj ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SPSB2_MOUSE SPSB2_MOUSE]] Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). | + | [https://www.uniprot.org/uniprot/SPSB2_MOUSE SPSB2_MOUSE] Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Babon, J J]] | + | [[Category: Mus musculus]] |
| - | [[Category: Masters, S L]] | + | [[Category: Babon JJ]] |
| - | [[Category: Nicholson, S E]] | + | [[Category: Masters SL]] |
| - | [[Category: Nicola, N A]] | + | [[Category: Nicholson SE]] |
| - | [[Category: Norton, R S]] | + | [[Category: Nicola NA]] |
| - | [[Category: Palmer, K R]] | + | [[Category: Norton RS]] |
| - | [[Category: Smith, B J]] | + | [[Category: Palmer KR]] |
| - | [[Category: Willson, T A]] | + | [[Category: Smith BJ]] |
| - | [[Category: Yao, S]] | + | [[Category: Willson TA]] |
| - | [[Category: Zhang, J G]] | + | [[Category: Yao S]] |
| - | [[Category: Beta sandwich]]
| + | [[Category: Zhang JG]] |
| - | [[Category: Gene regulation]]
| + | |
| Structural highlights
Function
SPSB2_MOUSE Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity).
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The four mammalian SPRY domain-containing SOCS box proteins (SSB-1 to SSB-4) are characterized by a C-terminal SOCS box and a central SPRY domain. We have determined the first SPRY-domain structure, as part of SSB-2, by NMR. This domain adopts a novel fold consisting of a beta-sandwich structure formed by two four-stranded antiparallel beta-sheets with a unique topology. We demonstrate that SSB-1, SSB-2 and SSB-4, but not SSB-3, bind prostate apoptosis response protein-4 (Par-4). Mutational analysis of SSB-2 loop regions identified conserved structural determinants for its interaction with Par-4 and the hepatocyte growth factor receptor, c-Met. Mutations in analogous loop regions of pyrin and midline-1 SPRY domains have been shown to cause Mediterranean fever and Opitz syndrome, respectively. Our findings provide a template for SPRY-domain structure and an insight into the mechanism of SPRY-protein interaction.
The SPRY domain of SSB-2 adopts a novel fold that presents conserved Par-4-binding residues.,Masters SL, Yao S, Willson TA, Zhang JG, Palmer KR, Smith BJ, Babon JJ, Nicola NA, Norton RS, Nicholson SE Nat Struct Mol Biol. 2006 Jan;13(1):77-84. Epub 2005 Dec 20. PMID:16369487[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Masters SL, Yao S, Willson TA, Zhang JG, Palmer KR, Smith BJ, Babon JJ, Nicola NA, Norton RS, Nicholson SE. The SPRY domain of SSB-2 adopts a novel fold that presents conserved Par-4-binding residues. Nat Struct Mol Biol. 2006 Jan;13(1):77-84. Epub 2005 Dec 20. PMID:16369487 doi:10.1038/nsmb1034
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