5xtu
From Proteopedia
(Difference between revisions)
| (3 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure of GDSL Esterase of Photobacterium sp. J15== | |
| + | <StructureSection load='5xtu' size='340' side='right'caption='[[5xtu]], [[Resolution|resolution]] 1.38Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5xtu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Photobacterium_sp._J15(2011) Photobacterium sp. J15(2011)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XTU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XTU FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.38Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xtu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xtu OCA], [https://pdbe.org/5xtu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xtu RCSB], [https://www.ebi.ac.uk/pdbsum/5xtu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xtu ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A0K0PV22_9GAMM A0A0K0PV22_9GAMM] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | GDSL esterase J15 (EstJ15) is a member of Family II of lipolytic enzyme. The enzyme was further classified in subgroup SGNH hydrolase due to the presence of highly conserve motif, Ser-Gly-Asn-His in four conserved blocks I, II, III, and V, respectively. X-ray quality crystal of EstJ15 was obtained from optimized formulation containing 0.10M ammonium sulphate, 0.15M sodium cacodylate trihydrate pH6.5, and 20% PEG 8000. The crystal structure of EstJ15 was solved at 1.38A with one molecule per asymmetric unit. The structure exhibits alpha/beta hydrolase fold and shared low amino acid sequence identity of 23% with the passenger domain of the autotransporter EstA of Pseudomonas aeruginosa. The active site is located at the centre of the structure, formed a narrow tunnel that hinder long substrates to be catalysed which was proven by the protein-ligand docking analysis. This study facilitates the understanding of high substrate specificity of EstJ15 and provide insights on its catalytic mechanism. | ||
| - | + | Crystallization and structure elucidation of GDSL esterase of Photobacterium sp. J15.,Mazlan SNHS, Ali MSM, Rahman RNZRA, Sabri S, Jonet MA, Leow TC Int J Biol Macromol. 2018 Nov;119:1188-1194. doi: 10.1016/j.ijbiomac.2018.08.022., Epub 2018 Aug 10. PMID:30102982<ref>PMID:30102982</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Jonet | + | <div class="pdbe-citations 5xtu" style="background-color:#fffaf0;"></div> |
| - | [[Category: Leow | + | == References == |
| - | [[Category: Mazlan | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Ali MSM]] | ||
| + | [[Category: Jonet MA]] | ||
| + | [[Category: Leow TC]] | ||
| + | [[Category: Mazlan SNHS]] | ||
| + | [[Category: Rahman RNZRA]] | ||
Current revision
Crystal Structure of GDSL Esterase of Photobacterium sp. J15
| |||||||||||
