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2i6y

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[[Image:2i6y.jpg|left|200px]]
 
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{{Structure
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==Structure and Mechanism of Mycobacterium tuberculosis Salicylate Synthase, MbtI==
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|PDB= 2i6y |SIZE=350|CAPTION= <scene name='initialview01'>2i6y</scene>, resolution 2.500&Aring;
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<StructureSection load='2i6y' size='340' side='right'caption='[[2i6y]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[2i6y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I6Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I6Y FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Anthranilate_synthase Anthranilate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.27 4.1.3.27] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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|GENE= mbtI, MT2454, Rv2386c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i6y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i6y OCA], [https://pdbe.org/2i6y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i6y RCSB], [https://www.ebi.ac.uk/pdbsum/2i6y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i6y ProSAT], [https://www.topsan.org/Proteins/TBSGC/2i6y TOPSAN]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i6y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i6y OCA], [http://www.ebi.ac.uk/pdbsum/2i6y PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2i6y RCSB]</span>
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[https://www.uniprot.org/uniprot/MBTI_MYCTU MBTI_MYCTU] Mediates the production of salicylate from chorismate via an isochorismate intermediate. Presents both isochorismate synthase and isochorismate-pyruvate lyase activities. Salycilate is the starter unit in the production of the virulence-conferring salicylate-based siderophore mycobactin.<ref>PMID:16923875</ref>
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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'''Structure and Mechanism of Mycobacterium tuberculosis Salicylate Synthase, MbtI'''
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i6/2i6y_consurf.spt"</scriptWhenChecked>
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==Overview==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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MbtI (rv2386c) from Mycobacterium tuberculosis catalyzes the initial transformation in mycobactin biosynthesis by converting chorismate to salicylate. We report here the structure of MbtI at 2.5 A resolution and demonstrate that isochorismate is a kinetically competent intermediate in the synthesis of salicylate from chorismate. At pH values below 7.5 isochorismate is the dominant product while above this pH value the enzyme converts chorismate to salicylate without the accumulation of isochorismate in solution. The salicylate and isochorismate synthase activities of MbtI are Mg2+-dependent, and in the absence of Mg2+ MbtI has a promiscuous chorismate mutase activity similar to that of the isochorismate pyruvate lyase, PchB, from Pseudomonas aeruginosa. MbtI is part of a larger family of chorismate-binding enzymes descended from a common ancestor (the MST family), that includes the isochorismate synthases and anthranilate synthases. The lack of active site residues unique to pyruvate eliminating members of this family, combined with the observed chorismate mutase activity, suggests that MbtI may exploit a sigmatropic pyruvate elimination mechanism similar to that proposed for PchB. Using a combination of structural, kinetic, and sequence based studies we propose a mechanism for MbtI applicable to all members of the MST enzyme family.
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==About this Structure==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i6y ConSurf].
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2I6Y is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I6Y OCA].
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<div style="clear:both"></div>
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== References ==
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==Reference==
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<references/>
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Structure and mechanism of MbtI, the salicylate synthase from Mycobacterium tuberculosis., Zwahlen J, Kolappan S, Zhou R, Kisker C, Tonge PJ, Biochemistry. 2007 Jan 30;46(4):954-64. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17240979 17240979]
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__TOC__
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[[Category: Anthranilate synthase]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Single protein]]
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[[Category: Kisker C]]
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[[Category: Kisker, C.]]
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[[Category: Subramaniapillai K]]
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[[Category: Subramaniapillai, K.]]
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[[Category: Tonge PJ]]
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[[Category: Tonge, P J.]]
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[[Category: Zhou R]]
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[[Category: Zhou, R.]]
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[[Category: Zwahlen J]]
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[[Category: Zwahlen, J.]]
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[[Category: beta sheet]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:40:34 2008''
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Current revision

Structure and Mechanism of Mycobacterium tuberculosis Salicylate Synthase, MbtI

PDB ID 2i6y

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