6g96
From Proteopedia
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==Crystal structure of TacT3 (tRNA acetylating toxin) from Salmonella== | ==Crystal structure of TacT3 (tRNA acetylating toxin) from Salmonella== | ||
| - | <StructureSection load='6g96' size='340' side='right' caption='[[6g96]], [[Resolution|resolution]] 1.48Å' scene=''> | + | <StructureSection load='6g96' size='340' side='right'caption='[[6g96]], [[Resolution|resolution]] 1.48Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6g96]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G96 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G96 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6g96]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_typhimurium"_loeffler_1892 "bacillus typhimurium" loeffler 1892]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G96 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G96 FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=BCN:BICINE'>BCN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=BCN:BICINE'>BCN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5fvj|5fvj]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5fvj|5fvj]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CU490_09610, CX046_09695, DD95_05355 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 "Bacillus typhimurium" Loeffler 1892])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g96 OCA], [http://pdbe.org/6g96 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g96 RCSB], [http://www.ebi.ac.uk/pdbsum/6g96 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g96 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g96 OCA], [http://pdbe.org/6g96 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g96 RCSB], [http://www.ebi.ac.uk/pdbsum/6g96 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g96 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Non-typhoidal Salmonella strains are responsible for invasive infections associated with high mortality and recurrence in sub-Saharan Africa, and there is strong evidence for clonal relapse following antibiotic treatment. Persisters are non-growing bacteria that are thought to be responsible for the recalcitrance of many infections to antibiotics. Toxin-antitoxin systems are stress-responsive elements that are important for Salmonella persister formation, specifically during infection. Here, we report the analysis of persister formation of clinical invasive strains of Salmonella Typhimurium and Enteritidis in human primary macrophages. We show that all the invasive clinical isolates of both serovars that we tested produce high levels of persisters following internalization by human macrophages. Our genome comparison reveals that S. Enteritidis and S. Typhimurium strains contain three acetyltransferase toxins that we characterize structurally and functionally. We show that all induce the persister state by inhibiting translation through acetylation of aminoacyl-tRNAs. However, they differ in their potency and target partially different subsets of aminoacyl-tRNAs, potentially accounting for their non-redundant effect. | ||
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| + | Activity of acetyltransferase toxins involved in Salmonella persister formation during macrophage infection.,Rycroft JA, Gollan B, Grabe GJ, Hall A, Cheverton AM, Larrouy-Maumus G, Hare SA, Helaine S Nat Commun. 2018 May 18;9(1):1993. doi: 10.1038/s41467-018-04472-6. PMID:29777131<ref>PMID:29777131</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6g96" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Bacillus typhimurium loeffler 1892]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Cheverton, A M]] | [[Category: Cheverton, A M]] | ||
[[Category: Gollan, B]] | [[Category: Gollan, B]] | ||
Current revision
Crystal structure of TacT3 (tRNA acetylating toxin) from Salmonella
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