5kj4

From Proteopedia

(Difference between revisions)

Current revision

Crystal Structure of Mouse Protocadherin-15 EC9-10

Structural highlights

5kj4 is a 4 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.35Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

PCD15_MOUSE Defects in Pcdh15 are the cause of the Ames waltzer (av) phenotype. It is characterized by deafness and a balance disorder, associated with the degeneration of inner ear neuroepithelia.

Function

PCD15_MOUSE Calcium-dependent cell-adhesion protein. Required for inner ear neuroepithelial cell elaboration and cochlear function. Probably involved in the maintenance of normal retinal function.

Publication Abstract from PubMed

Tip link filaments convey force and gate inner-ear hair-cell transduction channels to mediate perception of sound and head movements. Cadherin-23 and protocadherin-15 form tip links through a calcium-dependent interaction of their extracellular domains made of multiple extracellular cadherin (EC) repeats. These repeats are structurally similar, but not identical in sequence, often featuring linkers with conserved calcium-binding sites that confer mechanical strength to them. Here we present the X-ray crystal structures of human protocadherin-15 EC8-EC10 and mouse EC9-EC10, which show an EC8-9 canonical-like calcium-binding linker, and an EC9-10 calcium-free linker that alters the linear arrangement of EC repeats. Molecular dynamics simulations and small-angle X-ray scattering experiments support this non-linear conformation. Simulations also suggest that unbending of EC9-10 confers some elasticity to otherwise rigid tip links. The new structure provides a first view of protocadherin-15's non-canonical EC linkers and suggests how they may function in inner-ear mechanotransduction, with implications for other cadherins.

An elastic element in the protocadherin-15 tip link of the inner ear.,Araya-Secchi R, Neel BL, Sotomayor M Nat Commun. 2016 Nov 18;7:13458. doi: 10.1038/ncomms13458. PMID:27857071^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Araya-Secchi R, Neel BL, Sotomayor M. An elastic element in the protocadherin-15 tip link of the inner ear. Nat Commun. 2016 Nov 18;7:13458. doi: 10.1038/ncomms13458. PMID:27857071 doi:http://dx.doi.org/10.1038/ncomms13458

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5kj4"

Categories: Large Structures | Mus musculus | Araya-Secchi R | Sotomayor M

@@ Line 1: / Line 1: @@
 ==Crystal Structure of Mouse Protocadherin-15 EC9-10==
-<StructureSection load='5kj4' size='340' side='right' caption='[[5kj4]], [[Resolution|resolution]] 3.35&Aring;' scene=''>
+<StructureSection load='5kj4' size='340' side='right'caption='[[5kj4]], [[Resolution|resolution]] 3.35&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5kj4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KJ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KJ4 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5kj4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KJ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KJ4 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.35&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Pcdh15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kj4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kj4 OCA], [http://pdbe.org/5kj4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kj4 RCSB], [http://www.ebi.ac.uk/pdbsum/5kj4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kj4 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kj4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kj4 OCA], [https://pdbe.org/5kj4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kj4 RCSB], [https://www.ebi.ac.uk/pdbsum/5kj4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kj4 ProSAT]</span></td></tr>
 </table>
 == Disease ==
-[[http://www.uniprot.org/uniprot/PCD15_MOUSE PCD15_MOUSE]] Defects in Pcdh15 are the cause of the Ames waltzer (av) phenotype. It is characterized by deafness and a balance disorder, associated with the degeneration of inner ear neuroepithelia.
+[https://www.uniprot.org/uniprot/PCD15_MOUSE PCD15_MOUSE] Defects in Pcdh15 are the cause of the Ames waltzer (av) phenotype. It is characterized by deafness and a balance disorder, associated with the degeneration of inner ear neuroepithelia.
 == Function ==
-[[http://www.uniprot.org/uniprot/PCD15_MOUSE PCD15_MOUSE]] Calcium-dependent cell-adhesion protein. Required for inner ear neuroepithelial cell elaboration and cochlear function. Probably involved in the maintenance of normal retinal function.
+[https://www.uniprot.org/uniprot/PCD15_MOUSE PCD15_MOUSE] Calcium-dependent cell-adhesion protein. Required for inner ear neuroepithelial cell elaboration and cochlear function. Probably involved in the maintenance of normal retinal function.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 25: / Line 25: @@
 __TOC__
 </StructureSection>
-[[Category: Lk3 transgenic mice]]
+[[Category: Large Structures]]
-[[Category: Araya-Secchi, R]]
+[[Category: Mus musculus]]
-[[Category: Sotomayor, M]]
+[[Category: Araya-Secchi R]]
-[[Category: Adhesion]]
+[[Category: Sotomayor M]]
-[[Category: Calcium-binding protein]]
-[[Category: Hearing]]
-[[Category: Mechanotransduction]]

5kj4

From Proteopedia

Current revision

Crystal Structure of Mouse Protocadherin-15 EC9-10

Structural highlights

Disease

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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