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| - | [[Image:2i7z.gif|left|200px]] | |
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| - | {{Structure
| + | ==GAAA tetraloop receptor complex with associated manganese ions.== |
| - | |PDB= 2i7z |SIZE=350|CAPTION= <scene name='initialview01'>2i7z</scene>
| + | <StructureSection load='2i7z' size='340' side='right'caption='[[2i7z]]' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=MN6:MANGANESE+ION,+6+WATERS+COORDINATED'>MN6</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene>
| + | <table><tr><td colspan='2'>[[2i7z]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I7Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I7Z FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i7z OCA], [https://pdbe.org/2i7z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i7z RCSB], [https://www.ebi.ac.uk/pdbsum/2i7z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i7z ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=[[2adt|2ADT]], [[2i7e|2I7E]]
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i7z OCA], [http://www.ebi.ac.uk/pdbsum/2i7z PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2i7z RCSB]</span>
| + | __TOC__ |
| - | }}
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | '''GAAA tetraloop receptor complex with associated manganese ions.'''
| + | [[Category: Butcher SE]] |
| - | | + | [[Category: Davis JH]] |
| - | | + | |
| - | ==Overview==
| + | |
| - | Metal ions are critical for the proper folding of RNA, and the GAAA tetraloop-receptor is necessary for the optimal folding and function of many RNAs. We have used NMR to investigate the role of metal ions in the structure of the tetraloop-receptor in solution. The NMR data indicate native tertiary structure is formed under a wide range of ionic conditions. The lack of conformational adaptation in response to very different ionic conditions argues against a structural role for divalent ions. Nuclear Overhauser effects to cobalt hexammine and paramagnetic relaxation enhancement induced by manganese ions were used to determine the NMR structures of the tetraloop receptor in association with metal ions, providing the first atomic-level view of these interactions in the solution state. Five manganese and two cobalt hexammine ions could be localized to the RNA surface. The locations of the associated metal ions are similar, but not identical to, those of previously determined crystal structures. The sites of association are in general agreement with nonlinear Poisson-Boltzmann calculations of the electrostatic surface, emphasizing the general importance of diffusely associated ions in RNA tertiary structure.
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| - | | + | |
| - | ==About this Structure==
| + | |
| - | 2I7Z is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I7Z OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | Role of metal ions in the tetraloop-receptor complex as analyzed by NMR., Davis JH, Foster TR, Tonelli M, Butcher SE, RNA. 2007 Jan;13(1):76-86. Epub 2006 Nov 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17119098 17119098]
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| - | [[Category: Protein complex]] | + | |
| - | [[Category: Butcher, S E.]] | + | |
| - | [[Category: Davis, J H.]] | + | |
| - | [[Category: 11-nucleotide receptor]]
| + | |
| - | [[Category: gaaa tetraloop]]
| + | |
| - | [[Category: rna tertiary structure]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:40:56 2008''
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