|
|
| (3 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Solution Structure of a Thioredoxin-like Protein in the Oxidized Form== | | ==Solution Structure of a Thioredoxin-like Protein in the Oxidized Form== |
| - | <StructureSection load='2b5y' size='340' side='right' caption='[[2b5y]], [[NMR_Ensembles_of_Models | 11 NMR models]]' scene=''> | + | <StructureSection load='2b5y' size='340' side='right'caption='[[2b5y]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2b5y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B5Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B5Y FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2b5y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B5Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B5Y FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2b5x|2b5x]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 11 models</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b5y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b5y OCA], [http://pdbe.org/2b5y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2b5y RCSB], [http://www.ebi.ac.uk/pdbsum/2b5y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2b5y ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b5y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b5y OCA], [https://pdbe.org/2b5y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b5y RCSB], [https://www.ebi.ac.uk/pdbsum/2b5y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b5y ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/YKUV_BACSU YKUV_BACSU] Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| Line 12: |
Line 14: |
| | <jmolCheckbox> | | <jmolCheckbox> |
| | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b5/2b5y_consurf.spt"</scriptWhenChecked> | | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b5/2b5y_consurf.spt"</scriptWhenChecked> |
| - | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> | | </jmolCheckbox> |
| Line 30: |
Line 32: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Vibrio subtilis ehrenberg 1835]] | + | [[Category: Bacillus subtilis]] |
| - | [[Category: Jin, C]] | + | [[Category: Large Structures]] |
| - | [[Category: Xia, B]] | + | [[Category: Jin C]] |
| - | [[Category: Zhang, X]] | + | [[Category: Xia B]] |
| - | [[Category: Oxidoreductase]] | + | [[Category: Zhang X]] |
| - | [[Category: Thioredoxin-like]]
| + | |
| Structural highlights
Function
YKUV_BACSU Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The Bacillus subtilis YkuV responds to environmental oxidative stress and plays an important role for the bacteria to adapt to the environment. Bioinformatic analysis suggests that YkuV is a homolog of membrane-anchored proteins and belongs to the thioredoxin-like protein superfamily containing the typical Cys-Xaa-Xaa-Cys active motif. However, the biological function of this protein remains unknown thus far. In order to elucidate the biological function, we have determined the solution structures of both the oxidized and reduced forms of B. subtilis YkuV by NMR spectroscopy and performed biochemical studies. Our results demonstrated that the reduced YkuV has a low midpoint redox potential, allowing it to reduce a variety of protein substrates. The overall structures of both oxidized and reduced forms are similar, with a typical thioredoxin-like fold. However, significant conformational changes in the Cys-Xaa-Xaa-Cys active motif of the tertiary structures are observed between the two forms. In addition, the backbone dynamics provide further insights in understanding the strong redox potential of the reduced YkuV. Furthermore, we demonstrated that YkuV is able to reduce different protein substrates in vitro. Together, our results clearly established that YkuV may function as a general thiol:disulfide oxidoreductase, which acts as an alternative for thioredoxin or thioredoxin reductase to maintain the reducing environment in the cell cytoplasm.
The Bacillus subtilis YkuV is a thiol:disulfide oxidoreductase revealed by its redox structures and activity.,Zhang X, Hu Y, Guo X, Lescop E, Li Y, Xia B, Jin C J Biol Chem. 2006 Mar 24;281(12):8296-304. Epub 2006 Jan 17. PMID:16418167[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zhang X, Hu Y, Guo X, Lescop E, Li Y, Xia B, Jin C. The Bacillus subtilis YkuV is a thiol:disulfide oxidoreductase revealed by its redox structures and activity. J Biol Chem. 2006 Mar 24;281(12):8296-304. Epub 2006 Jan 17. PMID:16418167 doi:http://dx.doi.org/10.1074/jbc.M512015200
|
