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2bos

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A MUTANT SHIGA-LIKE TOXIN IIE BOUND TO ITS RECEPTOR

Structural highlights

2bos is a 5 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2Å
Ligands:	, , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q47644_ECOLX

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

BACKGROUND: Shiga-like toxins (SLTs) are produced by the pathogenic strains of Escherichia coli that cause hemorrhagic colitis and hemolytic uremic syndrome. These diseases in humans are generally associated with group II family members (SLT-II and SLT-IIc), whereas SLT-IIe (pig edema toxin) is central to edema disease of swine. The pentameric B-subunit component of the majority of family members binds to the cell-surface glycolipid globotriaosyl ceramide (Gb(3)), but globotetraosyl ceramide (Gb(4)) is the preferred receptor for SLT-IIe. A double-mutant of the SLT-IIe B subunit that reverses two sequence differences from SLT-II (GT3; Gln65-->Glu, Lys67-->Gln, SLT-I numbering) has been shown to bind more strongly to Gb(3) than to Gb(4). RESULTS: To understand the molecular basis of receptor binding and specificity, we have determined the structure of the GT3 mutant B pentamer, both in complex with a Gb(3) analogue (2.0 A resolution; R = 0.155, R(free) = 0.194) and in its native form (2.35 A resolution; R = 0.187, R(free) = 0.232). CONCLUSIONS: These are the first structures of a member of the medically important group II Shiga-like toxins to be reported. The structures confirm the previous observation of multiple binding sites on each SLT monomer, although binding site 3 is not occupied in the GT3 structure. Analysis of the binding properties of mutants suggests that site 3 is a secondary Gb(4)-binding site. The two mutated residues are located appropriately to interact with the extra betaGalNAc residue on Gb(4). Differences in the binding sites provide a molecular basis for understanding the tissue specificities and pathogenic mechanisms of members of the SLT family.

A mutant Shiga-like toxin IIe bound to its receptor Gb(3): structure of a group II Shiga-like toxin with altered binding specificity.,Ling H, Pannu NS, Boodhoo A, Armstrong GD, Clark CG, Brunton JL, Read RJ Structure. 2000 Mar 15;8(3):253-64. PMID:10745005^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ling H, Pannu NS, Boodhoo A, Armstrong GD, Clark CG, Brunton JL, Read RJ. A mutant Shiga-like toxin IIe bound to its receptor Gb(3): structure of a group II Shiga-like toxin with altered binding specificity. Structure. 2000 Mar 15;8(3):253-64. PMID:10745005

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2bos"

@@ Line 1: / Line 1: @@
 ==A MUTANT SHIGA-LIKE TOXIN IIE BOUND TO ITS RECEPTOR==
-<StructureSection load='2bos' size='340' side='right' caption='[[2bos]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+<StructureSection load='2bos' size='340' side='right'caption='[[2bos]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2bos]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BOS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BOS FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2bos]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BOS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BOS FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=NBU:N-BUTANE'>NBU</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bos OCA], [http://pdbe.org/2bos PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bos RCSB], [http://www.ebi.ac.uk/pdbsum/2bos PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2bos ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=NBU:N-BUTANE'>NBU</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bos OCA], [https://pdbe.org/2bos PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bos RCSB], [https://www.ebi.ac.uk/pdbsum/2bos PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bos ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q47644_ECOLX Q47644_ECOLX]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 28: / Line 31: @@
 ==See Also==
-*[[Shiga toxin|Shiga toxin]]
+*[[Shiga toxin 3D structures|Shiga toxin 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Bacillus coli migula 1895]]
+[[Category: Escherichia coli]]
-[[Category: Armstrong, G D]]
+[[Category: Large Structures]]
-[[Category: Boodhoo, A]]
+[[Category: Armstrong GD]]
-[[Category: Brunton, J L]]
+[[Category: Boodhoo A]]
-[[Category: Clark, C G]]
+[[Category: Brunton JL]]
-[[Category: Ling, H]]
+[[Category: Clark CG]]
-[[Category: Read, R J]]
+[[Category: Ling H]]
-[[Category: Protein-carbohydrate recognition]]
+[[Category: Read RJ]]
-[[Category: Receptor binding]]
-[[Category: Specificity]]
-[[Category: Toxin]]

2bos

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Current revision

A MUTANT SHIGA-LIKE TOXIN IIE BOUND TO ITS RECEPTOR

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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